FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343228
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC56440.42018183063485876No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG55110.4102803098208197No Hit
CCTATAATGGTCCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA55010.40953583457164383No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC52360.38980724046848336No Hit
GTCCAGGAGTGTTTGTTGCTCGTATCATAAATCAGTGTCCTGGGGGCTTG50650.37707671370757606No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48920.36419729189683364No Hit
CCATTATAGGAGAGCAAACAGAAATATTCCGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG45780.3408207690727114No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC44850.3338971492553759No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGGACCATTATAGGAGAGCAAACAGAAATATTCCG43570.32436786606592477No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42540.3166997709994133No Hit
GCCTGAGGATGAGGCGGAATATTTCTGTTTGCTCTCCTATAATGGTCCTCGGGTGTTCGGCGGAGGGACCAAG41890.31186068187977023No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA38270.2849106778596039No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36050.26838332732789966No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34780.2589284916633662No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG34600.2575884362148496No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33690.2508137114473492No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33430.24887807579949198No Hit
TTATAGGAGAGCAAACAGAAATATTCCGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC32200.23972103023462882No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31960.23793428963660673No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30700.2285539014969908No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30400.2263204757494632No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30140.22438484010160598No Hit
GTCCAATTCCCAGGCTGTAGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC29990.22326812722784217No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG29950.22297033712817185No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29850.22222586187899598No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT29370.2186523806829518No Hit
GTAGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC29070.2164189549354242No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28240.21023981036726452No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28070.20897420244366557No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27920.20785748956990177No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27850.20733635689547864No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27790.2068896717459731No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTTG27080.20160389747682447No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26330.1960203331080055No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26290.19572254300833514No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA26120.19445693508473616No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25530.19006453111459856No Hit
CTCCTATAATGGTCCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC24880.18522544199495544No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAG24770.184406519220862No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24680.1837364914966037No Hit
GCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAG24300.1809074855497354No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24160.17986522020088921No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22730.16921922413767432No Hit
CCCGAGGACCATTATAGGAGAGCAAACAGAAATATTCCGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGG22610.16832585383866328No Hit
GATTTATGATACGAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC22600.16825140631374572No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22520.167655826114405No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGGCGGAATATTTCTGTTTGCTC21610.16088110134690461No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC21590.16073220629706944No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC21290.15849878054954186No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21110.1571587251010253No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCGGAATATTTCTGTTTGCTCTCCTATAATGGTC20680.15395748152956906No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20500.1526174260810525No Hit
GCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCGGAATATTTCTG20340.15142626568237114No Hit
GAAATATTCCGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG20310.15120292310761835No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA20300.1511284755827008No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20190.15030955280860733No Hit
GAATATTTCTGTTTGCTCTCCTATAATGGTCCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG19920.1482994696358325No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18850.14033358446965072No Hit
GGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGG18730.1394402141706397No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18640.1387701864463814No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC18500.13772792109753518No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC18050.1343777824762438No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGGACCATTATAGGAGAGCAAACAGAAATATTCCGC17710.13184656662904587No Hit
CCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTTGCTCGTATCATAAATCAGTGTCCTGGGGGCTTGGCCAGG17700.13177211910412825No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17640.13132543395462276No Hit
CTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCC17570.13080430128019963No Hit
GCTTGGTCCCTCCGCCGAACACCCGAGGACCATTATAGGAGAGCAAACAGAAATATTCCGCCTCATCCTCAGG17530.13050651118052928No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17370.12931535078184792No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17040.12685858245956755No Hit
TCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTAGGGATAATGACCACTGGTGACAGCT16890.12574186958580374No Hit
GTCCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC16810.12514628938646305No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16610.12365733888811133No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16590.12350844383827615No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16530.12306175868877062No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16210.12067943789140786No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16150.12023275274190234No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15600.11613813887143508No Hit
CCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCGGAATATTTCTGTTTG15590.11606369134651749No Hit
GTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTAGGGATAATGACCACTGGTGACAGCTCCAGTGCTGGAG15520.11554255867209438No Hit
GCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGATACGAGCAACAAACACTCCTGGACCCCTGCCCGGTT15370.11442584579833058No Hit
GGCTTCTGCTGGAACCAGTAGGGATAATGACCACTGGTGACAGCTCCAGTGCTGGAGCCACAGGTGAGAGTGA15280.11375581807407231No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15170.11293689529997886No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15140.1127135527252261No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14950.11129904975179196No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14780.11003344182819298No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14720.10958675667868745No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14600.10869338637967642No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14510.10802335865541814No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC14220.10586438043280813No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14200.10571548538297296No Hit
GTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTG13880.10333316458561019No Hit
CAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGC13620.10139752893775295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)