FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343228
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG55000.4094613870467262No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC52310.3894350028438955No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC51680.3847448087740875No Hit
CCTATAATGGTCCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA50820.37834232163117504No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG48500.36107049585029494No Hit
GTCCAGGAGTGTTTGTTGCTCGTATCATAAATCAGTGTCCTGGGGGCTTG47510.3537001908834539No Hit
CCATTATAGGAGAGCAAACAGAAATATTCCGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG43230.3218366502187268No Hit
GCCTGAGGATGAGGCGGAATATTTCTGTTTGCTCTCCTATAATGGTCCTCGGGTGTTCGGCGGAGGGACCAAG39640.29510998877331324No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGGACCATTATAGGAGAGCAAACAGAAATATTCCG39510.2941421709493846No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA39060.2907920323280932No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC38470.2863996283579556No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37790.2813371966635597No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35380.2633953431584214No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35170.26183194513515207No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32100.23897655498545295No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31830.23696647181267813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31640.23555196883924398No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31540.2348074935900681No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31400.2337652282412219No Hit
TTATAGGAGAGCAAACAGAAATATTCCGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC30100.22408705000193566No Hit
GTCCAATTCCCAGGCTGTAGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC29810.22192807177932564No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG29130.21686564008492976No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28350.21105873314135798No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28340.2109842856164404No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28050.20882530739383037No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27350.20361398064959935No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27100.20175279252665967No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26300.19579699053325272No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC25620.19073455883885684No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25520.18999008358968097No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25140.18716107764281267No Hit
GTAGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC25090.18678884001822477No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24680.1837364914966037No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTTG24570.18291756872251025No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA24220.18031190535039474No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAG23800.1771851093038561No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23660.17614284395500987No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23360.17390941820748226No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22990.17115485978553155No Hit
CTCCTATAATGGTCCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC22920.17063372711110847No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21970.16356121224393774No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT21730.16177447164591566No Hit
GATTTATGATACGAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC21620.1609555488718222No Hit
GCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAG21490.15998773104789357No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21240.15812654292495393No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21080.15693538252627254No Hit
CCCGAGGACCATTATAGGAGAGCAAACAGAAATATTCCGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGG20920.15574422212759115No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC20910.15566977460267356No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20630.15358524390498113No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20510.15269187360597009No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20380.15172405578204146No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGGCGGAATATTTCTGTTTGCTC19810.14748054686173903No Hit
GGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGG19510.14524712111421142No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19140.14249256269226074No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19060.14189698249292004No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCGGAATATTTCTGTTTGCTCTCCTATAATGGTC18980.14130140229357935No Hit
GCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCGGAATATTTCTG18790.1398868993201452No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18400.1369834458483593No Hit
GAAATATTCCGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG18150.13512225772541966No Hit
GAATATTTCTGTTTGCTCTCCTATAATGGTCCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG18030.13422888742640862No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17100.12730526760907307No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC17050.12693302998448514No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA16970.12633744978514444No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16570.12335954878844098No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC16510.12291286363893546No Hit
CCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTTGCTCGTATCATAAATCAGTGTCCTGGGGGCTTGGCCAGG16410.12216838838975959No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16240.12090278046616061No Hit
GTCCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC16220.12075388541632544No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16020.11926493491797373No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15930.11859490719371543No Hit
CTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCC15880.1182226695691275No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGGACCATTATAGGAGAGCAAACAGAAATATTCCGC15670.11665927154585819No Hit
GCTTGGTCCCTCCGCCGAACACCCGAGGACCATTATAGGAGAGCAAACAGAAATATTCCGCCTCATCCTCAGG15530.11561700619701198No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15470.11517032104750645No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15430.11487253094783612No Hit
TCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTAGGGATAATGACCACTGGTGACAGCT15370.11442584579833058No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGG15110.11249021015047334No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15040.11196907747605023No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14980.11152239232654472No Hit
CCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCGGAATATTTCTGTTTG14890.11085236460228642No Hit
GTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTAGGGATAATGACCACTGGTGACAGCTCCAGTGCTGGAG14480.10780001608066538No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC14280.10631106558231367No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC14140.10526880023346744No Hit
GCAGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGATACGAGCAACAAACACTCCTGGACCCCTGCCCGGTT14000.10422653488462122No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC13950.10385429726003328No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG13480.10035526358890673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)