FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834393
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27460.3291015145141438No Hit
CATCTATAGTGTGTCCAGCAGGGCCGCTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA25750.30860757460812827No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22970.2752899413106294No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22390.2683387804068347No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21070.25251889697061214No Hit
ACTATATACTACTCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGA20680.24784484050081917No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAAAATTTCCTGGTAAGAGGCTGA19790.2371784039415479No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19590.23478145190575667No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACAA19290.23118602385206968No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19050.22830968140912017No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18750.22471425335543324No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18520.22195775851427324No Hit
GTATATAGTGGTCCCACCACCACTAATGTATGCAATCCACTCCGGAGCCTTCCCTGGAGCCTGGCGGATCCAACT17170.20577833227268205No Hit
AGCCTGGAGGGTCCCTGAGACTTTCCTGTGCAGGCTCTGGATTCAAGTTC17160.20565848467089248No Hit
CTCTTGTAGGGCCAGTCAGCCTCTTACCAGGAAATTTTTAGCCTGGTATC16970.20338138023689076No Hit
TCTATAGTGTGTCCAGCAGGGCCGCTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16000.19175616286330302No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15690.18804088720782652No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15300.18336683073803353No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGGAGCCTGAAGATT14670.17581643182529097No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13900.1665881664874945No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13780.16514999526601973No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13630.16335228123917628No Hit
CATTAGTGGTGGTGGGACCACTATATACTACTCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACCC13420.16083548160159541No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13200.15819883436222498No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13190.1580789867604354No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13000.15580188232643372No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12770.15304538748527374No Hit
GCCCCAGACTTCCCAGGAGTTTCCAATCGGTGATGAGCTCTGAACACAGA12740.15268584467990504No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12670.15184691146737808No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG12550.1504087402459033No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12000.14381712214747724No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11820.14165986531526512No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11780.14118047490810684No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11440.13710565644726166No Hit
CCACTAATGTATGCAATCCACTCCGGAGCCTTCCCTGGAGCCTGGCGGATCCAACTCATGTAGAAGTCACTGAA11430.1369858088454721No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11420.13686596124368253No Hit
GTCTGGGGGACACTTGGTCAAGCCTGGAGGGTCCCTGAGACTTTCCTGTGCAGGCTCTGGATTCAAGTTCAGTGA11070.13267129518104778No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10910.13075373355241474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10770.12907586712736085No Hit
GGGATGTAGTAGGTCCCACTATACGTGTCTCTCACACAGAAATAGACGGC10740.12871632432199218No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGCGGCCCTGCT10660.12775754350767565No Hit
TCCCAGGAGTTTCCAATCGGTGATGAGCTCTGAACACAGAGGACTCACCATGGAGCTTGGGCTGAGCTGGGTTTT10630.12739800070230695No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10630.12739800070230695No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10400.12464150586114697No Hit
CACTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAAAATTTCCTGGTAAGAGG10320.12368272504683045No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10240.12272394423251394No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10060.12056668740030177No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACCGAGCCACCCTCTCTT10030.12020714459493309No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10010.11996744939135395No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA9890.11852927816987918No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9750.11685141174482527No Hit
GACTTCACTCTCACCATCACCAGCCTGGAGCCTGAAGATTCTGCAGTTTATTATTGTCAGCAACATGCTGGTTCT9720.11649186893945658No Hit
CTCCAGGGACAACCCCAAGGAGTCACTGTATCTGGACTTGAGCAGCCTGAGAGTCGAGGACACGGCCGTCTATTT9670.11589263093050876No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC9500.11385522170008616No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA9500.11385522170008616No Hit
GAAATAGACGGCCGTGTCCTCGACTCTCAGGCTGCTCAAGTCCAGATACA9470.11349567889471748No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9410.11277659328398008No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9400.11265674568219054No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC9320.11169796486787402No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9220.11049948884997837No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG9180.1100200984428201No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9170.10990025084103054No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC9160.10978040323924099No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG9070.10870177482313491No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9030.10822238441597663No Hit
CCATGGAGCTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTATAAAAGGT8980.10762314640702883No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8850.10606512758376449No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8750.10486665156586884No Hit
CTATACGTGTCTCTCACACAGAAATAGACGGCCGTGTCCTCGACTCTCAGGCTGCTCAAGTCCAGATACAGTGA8740.10474680396407927No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8730.1046269563622897No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA8720.10450710876050015No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACCGAGCCACCCTCTCTTGT8650.1036681755479732No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8640.10354832794618363No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA8610.10318878514081495No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8610.10318878514081495No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAC8490.10175061391934016No Hit
GTCTTTGTCTCCAGGGGACCGAGCCACCCTCTCTTGTAGGGCCAGTCAGC8390.10055213790144452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACA200.00679697952.79892361
ACGAGAC1100.046.8452322
CTCGTAT950.043.64726639
CTTATAC1300.042.270681
ATGCCGT1250.041.5852644
CGTATGC1250.041.52248841
CGAGACG1250.041.2287423
GTATCAA12700.040.5648771
TCGTATG1250.038.7356140
TATACAC1550.037.6686333
ATCTCGT1100.037.64996337
CATCTCG1100.037.6295736
TGCCGTC1400.037.15440845
TGTTAGA5800.036.69279570
GACGTCG400.002646119634.36552425
ACTATCG400.0026531334.346999
ATCTCCG1500.034.3469910
ATAAGCC400.002653909834.344933
TATCAAC15500.033.0154462
TCAACGC15850.032.5030864
Produced by FastQC (version 0.11.5)