FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834393
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26560.318315230353083No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23880.2861960730734798No Hit
CATCTATAGTGTGTCCAGCAGGGCCGCTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23580.2826006450197928No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21630.2592303626708278No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20840.24976240212945222No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAAAATTTCCTGGTAAGAGGCTGA19220.23034709063954276No Hit
ACTATATACTACTCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGA18740.22459440575364367No Hit
CTCTTGTAGGGCCAGTCAGCCTCTTACCAGGAAATTTTTAGCCTGGTATC16750.20074473299752038No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACAA16530.19810808575814995No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16510.1978683905545708No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15940.19103707725256563No Hit
GTATATAGTGGTCCCACCACCACTAATGTATGCAATCCACTCCGGAGCCTTCCCTGGAGCCTGGCGGATCCAACT15720.18840043001319523No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15430.18492484956129784No Hit
AGCCTGGAGGGTCCCTGAGACTTTCCTGTGCAGGCTCTGGATTCAAGTTC15350.18396606874698135No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15150.18156911671119005No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14550.1743782606038162No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14490.1736591749930788No Hit
TCTATAGTGTGTCCAGCAGGGCCGCTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14150.1695843565322336No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14080.16874542331970666No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGGAGCCTGAAGATT13690.16407136684991364No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13370.1602362435926476No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13180.15795913915864584No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12790.15328508268885285No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12610.1511278258566407No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12540.15028889264411374No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12460.14933011182979725No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11870.14225910332421293No Hit
GCCCCAGACTTCCCAGGAGTTTCCAATCGGTGATGAGCTCTGAACACAGA11610.13914306567768425No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11420.13686596124368253No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11330.13578733282757646No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11240.13470870441147037No Hit
CATTAGTGGTGGTGGGACCACTATATACTACTCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACCC11020.13207205717209997No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG10830.1297949527380982No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10650.12763769590588608No Hit
GGGATGTAGTAGGTCCCACTATACGTGTCTCTCACACAGAAATAGACGGC10600.12703845789693824No Hit
TCCCAGGAGTTTCCAATCGGTGATGAGCTCTGAACACAGAGGACTCACCATGGAGCTTGGGCTGAGCTGGGTTTT10540.12631937228620085No Hit
GTCTGGGGGACACTTGGTCAAGCCTGGAGGGTCCCTGAGACTTTCCTGTGCAGGCTCTGGATTCAAGTTCAGTGA10280.12320333463967219No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10240.12272394423251394No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10110.12116592540924961No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10040.12032699219672263No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGCGGCCCTGCT9970.11948805898419569No Hit
CCACTAATGTATGCAATCCACTCCGGAGCCTTCCCTGGAGCCTGGCGGATCCAACTCATGTAGAAGTCACTGAA9960.11936821138240612No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG9900.11864912577166874No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9860.11816973536451049No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9720.11649186893945658No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC9620.11529339292156095No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9460.11337583129292791No Hit
CACTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAAAATTTCCTGGTAAGAGG9450.11325598369113835No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9400.11265674568219054No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9370.11229720287682184No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9350.1120575076732427No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9310.11157811726608445No Hit
CTCCAGGGACAACCCCAAGGAGTCACTGTATCTGGACTTGAGCAGCCTGAGAGTCGAGGACACGGCCGTCTATTT9280.11121857446071576No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9210.1103796412481888No Hit
CCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCTGCCACCTGCT9120.10930101283208273No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9120.10930101283208273No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9080.10882162242492448No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC9060.10858192722134534No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9030.10822238441597663No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8990.1077429940088184No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8930.10702390839808101No Hit
GACTTCACTCTCACCATCACCAGCCTGGAGCCTGAAGATTCTGCAGTTTATTATTGTCAGCAACATGCTGGTTCT8800.10546588957481666No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA8530.10223000432649844No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8520.10211015672470887No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT8480.1016307663175506No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8390.10055213790144452No Hit
GAAATAGACGGCCGTGTCCTCGACTCTCAGGCTGCTCAAGTCCAGATACA8370.1003124426978654No Hit
CCATGGAGCTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTATAAAAGGT8360.10019259509607584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGT350.001169964440.57838465
GTATCAA16200.039.162661
TATCAAC18200.034.8465232
TTAAGCA400.002687558534.2567869
ATCAACG18700.033.7132763
TCAACGC18700.033.526034
AACGCAG19100.032.8239146
CAACGCA19300.032.6612785
TATTAGA553.3798898E-431.164952
CGCAGAG20200.031.0364768
AGAGTAC20750.030.2065811
GCAGAGT21800.028.5997039
CCTAAAC756.4130174E-527.43832422
GGTACCT500.00796561527.43338620
AATCCGG500.00798662727.4185815
CGGGATT500.00800299927.4070744
TACACAC500.00800299927.4070745
GACCTAA658.975975E-426.37350818
CAGAGTA24100.025.86562210
GGATCCG7200.025.5898270
Produced by FastQC (version 0.11.5)