FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770540
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACGCGGGAGTCCGGGGTCCCT38500.4996495963869494No Hit
GTTTTATACAGCTCCGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA36670.47590001816907623No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34270.44475303034235736No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32220.4181483115737015No Hit
GTTTAGGAGGCTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGTATAAAACATT27720.35974770939860357No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21340.27694863342590914No Hit
GTAATATAGGGCCGTGTCCTCGGCTGTCAGGCTGTTCAATTGCAGATACA21110.2739637137591819No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTGTCAGGCTGTTCAATTGCAGATA20000.25955823188932436No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19960.25903911542554575No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19850.25761154515015444No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19470.25267993874425726No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19010.2467100994108028No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18710.24281672593246295No Hit
GTATAAAACATTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACATAGCCAGGGAGTCTGGAGA17350.2251667661639889No Hit
AGCCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACGCGGGAG16930.21971604329431307No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16820.21828847301892182No Hit
GTTTATTACTGTCAGCAATATTATGATAATCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA16680.2164715653956965No Hit
ATATTACTGTGTGAGAGGGGGGAAGGGTAGTGGTAGAAAGCCTTTTGACTACTGGGGCCAGGGAACCCTGGTCAC16600.21543333246813925No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16180.20998260959846343No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15980.20738702727957017No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15910.20647857346795753No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15810.20518078230851092No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15480.20089807148233707No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15120.19622602330832922No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14780.1918135333662107No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14690.19064552132270876No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14680.1905157422067641No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14090.18285877436602901No Hit
GTCTCCAGACTCCCTGGCTATGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAATGTTTTATA14060.18246943701819504No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13240.17182754951073273No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13230.17169777039478806No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13180.17104887481506476No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC12520.16248345316271706No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGCTGC12280.15936875438004516No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12250.15897941703221116No Hit
GATTTCACTCTCACCATCAGCAGCCTACAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATAAT12240.15884963791626652No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11920.15469670620603734No Hit
GTAATAAACTGCCACATCTTCAGCCTGTAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA11910.15456692709009268No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11760.15262024035092273No Hit
GGCTATGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGA11740.15236068211903342No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11710.15197134477119942No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11310.14678018013341293No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11090.14392503958263037No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10870.1410698990318478No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10810.14029122433617983No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG10590.13743608378539723No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10510.13639785085783995No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10080.1308173488722195No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC9930.12887066213304954No Hit
GCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTATGTCTCTGGGCGAGAGGGCCACCATCAA9730.1262750798141563No Hit
GTCAGGGACCCCGGACTCCCGCGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGCTTCTGCTGGTA9660.12536662600254367No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9600.12458795130687571No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9430.12238170633581645No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9430.12238170633581645No Hit
ATATTATGATAATCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9410.12212214810392712No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9270.12030524048070185No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8930.11589275053858333No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATAATCTGT8930.11589275053858333No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8910.115633192306694No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8900.11550341319074935No Hit
ATGATAATCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA8830.1145949593791367No Hit
GCTCATTTACTGGGCATCTACGCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT8750.11355672645157941No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8690.11277805175591143No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAATGTTTTATACAGCTCCGACAATAAGAACTA8680.11264827263996677No Hit
CCTTGGTCCCTTGGCCGAACGTCCACAGATTATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC8660.11238871440807745No Hit
GCCTACAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATAATCTGTGGACGTTCGGCCAAGGGA8610.11173981882835414No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8610.11173981882835414No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8540.1108313650167415No Hit
ATCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATCTG8500.11031224855296287No Hit
GCCCTATATTACTGTGTGAGAGGGGGGAAGGGTAGTGGTAGAAAGCCTTT8490.11018246943701819No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8490.11018246943701819No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8430.10940379474135022No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8420.10927401562540555No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT8370.10862512004568224No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8300.10771666623406963No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8260.10719754977029097No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT8230.10680821242245699No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8220.1066784333065123No Hit
TCTTCAGCCTGTAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC8190.10628909595867832No Hit
CTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACGCGGGAGTC8160.10589975861084433No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8040.10434240921950838No Hit
ATATAGGGCCGTGTCCTCGGCTGTCAGGCTGTTCAATTGCAGATACAGTG8040.10434240921950838No Hit
CTCCTGTGCCGCCTCTGGATTCACCTTTAGTAGCTATGTCATGAGTTGGGTCCGCCAGGCGCCAGGGAAGGGA8000.10382329275572975No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7940.10304461806006178No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG7900.10252550159628313No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT7740.10044903574116854No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC7720.10018947750927922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)