FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770540
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACGCGGGAGTCCGGGGTCCCT34000.4412489942118515No Hit
GTTTTATACAGCTCCGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA32820.42593505853038127No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32250.4185376489215355No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26780.34754847249980536No Hit
GTTTAGGAGGCTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGTATAAAACATT24660.32003529991953694No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22660.2940794767306045No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21060.2733148181794586No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20010.259688011005269No Hit
GTAATATAGGGCCGTGTCCTCGGCTGTCAGGCTGTTCAATTGCAGATACA19020.2468398785267475No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18910.24541230825135618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18300.2374957821787318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17990.23347262958444726No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17670.2293196978742181No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTGTCAGGCTGTTCAATTGCAGATA17360.22529654527993354No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16980.22036493887403638No Hit
GTTTATTACTGTCAGCAATATTATGATAATCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA16690.21660134451164118No Hit
ATATTACTGTGTGAGAGGGGGGAAGGGTAGTGGTAGAAAGCCTTTTGACTACTGGGGCCAGGGAACCCTGGTCAC15800.20505100319256625No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15650.2031043164533963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14900.19337088275754666No Hit
AGCCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACGCGGGAG14900.19337088275754666No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14680.1905157422067641No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14420.18714148519220286No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGCTGC14340.18610325226464558No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14070.18259921613413968No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14050.18233965790225037No Hit
GTAATAAACTGCCACATCTTCAGCCTGTAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13910.1805227502790251No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13630.17688893503257458No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13500.17520180652529393No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13360.17338489890206868No Hit
GTATAAAACATTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACATAGCCAGGGAGTCTGGAGA13330.1729955615542347No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13300.1726062242064007No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12940.16793417603239286No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12940.16793417603239286No Hit
GTCTCCAGACTCCCTGGCTATGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAATGTTTTATA12800.1661172684091676No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12500.16222389493082773No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12160.15781140498870921No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC11950.15508604355387132No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11940.15495626443792665No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11670.15145222830742078No Hit
GATTTCACTCTCACCATCAGCAGCCTACAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATAAT11460.14872686687258285No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11370.1475588548290809No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10940.14197835284346041No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10840.1406805616840138No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10210.1325044773795001No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10110.13120668622005346No Hit
GGCTATGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGA10090.13094712798816416No Hit
TCTTCAGCCTGTAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC10080.1308173488722195No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG9800.12718353362576895No Hit
ATATTATGATAATCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9480.12303060191553976No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9440.1225114854517611No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9430.12238170633581645No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9360.12147325252420381No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9320.12095413606042515No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9280.12043501959664651No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATAATCTGT9180.11913722843719989No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9150.1187478910893659No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9060.11757987904586394No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9050.11745009992991928No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC8970.11641186700236197No Hit
ATGATAATCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA8890.11537363407480468No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG8880.11524385495886003No Hit
CCTTGGTCCCTTGGCCGAACGTCCACAGATTATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC8720.11316738910374542No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGACTCCCGCGT8720.11316738910374542No Hit
GTCAGGGACCCCGGACTCCCGCGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGCTTCTGCTGGTA8580.11135048148052015No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8570.11122070236457549No Hit
GCCTACAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATAATCTGTGGACGTTCGGCCAAGGGA8550.11096114413268617No Hit
CTCCTGTGCCGCCTCTGGATTCACCTTTAGTAGCTATGTCATGAGTTGGGTCCGCCAGGCGCCAGGGAAGGGA8540.1108313650167415No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8540.1108313650167415No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8520.11057180678485218No Hit
GCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTATGTCTCTGGGCGAGAGGGCCACCATCAA8460.1097931320891842No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8450.10966335297323954No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC8380.10875489916162692No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8320.10797622446595895No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGTAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA8250.10706777065434629No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8170.10602953772678901No Hit
ATATAGGGCCGTGTCCTCGGCTGTCAGGCTGTTCAATTGCAGATACAGTG8160.10589975861084433No Hit
CACCATCAGCAGCCTACAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATAATCTGTGGACGTT8090.10499130479923172No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8020.10408285098761907No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8000.10382329275572975No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7810.10135748955278116No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7810.10135748955278116No Hit
GCCCTATATTACTGTGTGAGAGGGGGGAAGGGTAGTGGTAGAAAGCCTTT7730.10031925662522387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)