EGAF00004688623 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004688623
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808718
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	2343	0.28971780027154087	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	2156	0.26659478334846015	No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG	1668	0.20625236485400353	No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	1558	0.19265059019336778	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC	1510	0.18671527034145402	No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	1418	0.17533924062528594	No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA	1357	0.16779643831347885	No Hit
GATCTATGCTACATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA	1341	0.16581799836284095	No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG	1330	0.16445782089677738	No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG	1321	0.16334494842454353	No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA	1313	0.16235572844922458	No Hit
GTAGGAGACAGAGTCACCATGACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAA	1287	0.15914076352943796	No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG	1186	0.14665186134103608	No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC	1179	0.14578629386263198	No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATGACTT	1150	0.14220037145210074	No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT	1094	0.135275831624868	No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT	1082	0.13379200166188956	No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT	1081	0.1336683491649747	No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG	1079	0.13342104417114495	No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC	1076	0.13305008668040033	No Hit
GGCCAAAAGTGTACGGGTAACTAAAATGCTGGAGACAGAAATAAGTTGCA	1061	0.13119529922667728	No Hit
ATGCTACATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA	1047	0.1294641642698691	No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG	1037	0.1282276393007204	No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC	1023	0.1264965043439122	No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT	988	0.12216866695189176	No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG	928	0.11474951713699956	No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC	925	0.11437855964625493	No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT	924	0.11425490714934007	No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC	923	0.11413125465242518	No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA	915	0.11314203467710623	No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG	904	0.11178185721104265	No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	885	0.10943245976966012	No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTTCTGTCTCCAGCATTTTAGTTAC	875	0.10819593480051143	No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT	868	0.10733036732210734	No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT	825	0.10201330995476793	No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT	819	0.1012713949732787	No Hit
CTAAAATGCTGGAGACAGAAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC	817	0.10102408997944895	No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT	816	0.10090043748253409	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAA	20	0.0065012695	53.395844	58
AACGCCG	20	0.006997528	52.411934	48
CGAGACG	70	9.094947E-12	48.588573	23
CGTATGC	75	1.6370905E-11	46.078934	41
TATCTCG	75	1.8189894E-11	45.72833	36
TACGTAT	85	2.3725588E-6	44.397682	70
ATCTCGT	80	3.6379788E-11	42.92653	37
GGTACGT	55	3.2428325E-6	42.431564	68
TCGTATG	85	6.548362E-11	40.60174	40
ACGAGAC	85	7.8216544E-11	40.011642	22
ATCTCCG	90	1.4370016E-10	37.78176	10
TGGTACG	60	7.905059E-6	37.269127	67
CGGACTC	95	2.5283953E-10	35.839787	28
TATACAC	105	2.0008883E-11	35.6206	3
GTATCAA	1910	0.0	35.4322	1
GTACGTA	75	1.3733174E-5	34.382557	69
GACGGAC	100	4.4019544E-10	34.03305	26
ATGCCGT	105	6.039045E-10	33.050613	44
CCCACGA	105	7.4942363E-10	32.388374	19
CTTATAC	105	7.4942363E-10	32.388374	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content