FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502947
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58250.3875718837723486No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36060.23992862023744016No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGACAAGGTTACTCCTACTGTCCCGGGAG35960.23926326078032029No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC34870.23201084269771324No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31490.2095216930470602No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30260.2013377717244853No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC29730.19781136660174978No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28880.19215581121623052No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28580.19015973284487078No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT28480.18949437338775088No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27150.18064509260805603No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25430.16920090994559356No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24580.1635453545600743No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23970.15948666187164284No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT23850.15868823052309897No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22900.15236731568045978No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC22680.150903524874796No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21990.1463125446206686No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT21740.1446491459778688No Hit
GACTATTACTGTAGCTCCCGGGACAGTAGGAGTAACCTTGTCCTATTCGG21030.13992509383231744No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20530.13659829654671787No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20460.13613254492673396No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20440.13599947303530996No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC20120.13387032277252625No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19470.12954548630124682No Hit
AGCCAGGACAGGCCCCTGTATTCGTCACTTATGGTAACAATCAATGGCCCTCAGCGCTCCCGGACCGGTTCTCTG19300.12841437522414298No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19270.128214767387007No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18170.12089581335868796No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17910.11916587877017619No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG17800.1184339833673443No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17790.11836744742163229No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17660.11750248012737641No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCACGATCACATGCCAAGGAGACAGCCTCAGAGA17590.11703672850739248No Hit
GTAATAGTCAGCCTCATCGTCCGCCTGAGTCCCAGTGATGGTCAACGAAG17470.11623829715884859No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT17440.11603868932171262No Hit
CTATTACTGTAGCTCCCGGGACAGTAGGAGTAACCTTGTCCTATTCGGCG17440.11603868932171262No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17360.11550640175601667No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17300.11510718608174474No Hit
GTAATACACGGCCGTGTCACCGGCGGTCACAGAGCGCAATCTCAGGGCAA16960.11284496392753704No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16570.11025006204476938No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16100.10712287259630579No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT16090.1070563366505938No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA15920.10592522557348995No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15820.10525986611637005No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA15500.10313071585358632No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15500.10313071585358632No Hit
AGGTTACTCCTACTGTCCCGGGAGCTACAGTAATAGTCAGCCTCATCGTC15500.10313071585358632No Hit
ACCTTGTCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCC15470.10293110801645035No Hit
GGACAAGGTTACTCCTACTGTCCCGGGAGCTACAGTAATAGTCAGCCTCA15150.10080195775366663No Hit
GAGTAACCTTGTCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA15090.10040274207939467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3650.047.18999540
CGTATGC3950.043.70339641
GGTATCA18150.043.3785251
TCTCGTA3600.041.9480838
AGGGGGG5500.040.84155770
ATCTCGT3700.040.74577337
ATGCCGT4300.040.33598344
TATCTCG3650.040.30426436
TAATGAT5050.039.24785270
ACGAGAC4550.038.0282722
GCCGTCT4600.037.8521746
GAGCCTA4450.037.49149731
GCCTATC4100.037.4464733
ATCTCCG4550.036.5333210
GAGACCG4650.036.4881324
CACGAGA4850.036.3731221
GTATCAA43350.036.010751
GTATGCC5000.035.97248542
CCGTCTT4900.035.60521747
AGACCGG4800.035.35493525
Produced by FastQC (version 0.11.5)