FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177931
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGATATCACTATCTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATT60860.5166686333919389No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49090.41674767027949855No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43860.37234778607575486No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGGGCTGGATCCG34410.2921223738911702No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33060.2806616007219438No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30920.2624941528833183No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCACGTCTAGTCAGAGCCTCCTG30460.25858900054417444No Hit
CTACAAACTCCTCGGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT30250.2568062136067393No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGA29880.2536651128122106No Hit
GTGTAAAATCTGTGCCTGATCGACTGCCACTGAACCTGTCAGGGACCCCG29450.2500146443212718No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28490.24186476117871084No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28400.24110070963409572No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28220.2395726065448655No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAGA27970.23745024114315694No Hit
GATTTTACACTGAGAATTAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT26900.22836651722384416No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATTCTCAGTGTAAAATCTGTGCCTGATCGACTGCCA26070.22132026409017166No Hit
ATCCATTACTGTGCAGGAGGCTCTGACTAGACGTGCAGGAGATGGAGGCC25460.2161416925100027No Hit
GGCCAAAAGTGGCCCGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTG25020.21240632940299561No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24640.20918033399239855No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24220.2056147601175281No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24170.2051902870371864No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23680.20103045084983753No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23540.19984192622488073No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23340.19814403390351387No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23180.19678572004642036No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATTCTCAGTGTAAAAT23050.19568209003753192No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22560.19152225385018307No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22450.1905884130734313No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22380.18999415076095288No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21370.1814197945380502No Hit
GTTCAGTGGCAGTCGATCAGGCACAGATTTTACACTGAGAATTAGCAGAG20910.1775146421989064No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20490.17394906832403595No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGG20440.17352459524369424No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20400.17318501677942086No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG20240.17182670292232735No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTGCTCGGGCC19790.16800644519925192No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19630.16664813134215842No Hit
GAATTAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAA19630.16664813134215842No Hit
CTCCTGCACAGTAATGGATATCACTATCTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT19510.1656293959493383No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19040.16163934899412613No Hit
GTATTATTGTGCGGGGCTCTTCATTTCCCGGCGATACTACGGGGTGCAAGTCTGGGGCCAAGGGACCACGGTCAC18840.15994145667275927No Hit
GTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCACGTCTAGTCAGAGCCTCCTGCA18690.15866803743173413No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18680.15858314281566577No Hit
GGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTGCTCGGGCCTCCGGGGT17690.15017857582489974No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGA17010.14440574193225242No Hit
GTCCCTGACAGGTTCAGTGGCAGTCGATCAGGCACAGATTTTACACTGAG16930.14372658500370564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16920.1436416903876373No Hit
CGTCTAGTCAGAGCCTCCTGCACAGTAATGGATATCACTATCTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTC16860.14313232269122725No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16840.14296253345909057No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCC16820.14279274422695387No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCC16620.14109485190558702No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16620.14109485190558702No Hit
GGATATCACTATCTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTGCT16410.1393120649681518No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16390.1391422757360151No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCGGGCCACTTTTGGCCA16090.1365954372539648No Hit
ATTACAACCCGTCCCTCCAGAGTCGAGTCACCATATTCGTGGACACGTCCAAGAGCCAGTTCTCCCTGAAGTTGA15970.13557670186114468No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15740.13362412569157275No Hit
GTCAGGGACCCCGGAGGCCCGAGCAGAATCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCACGTA15610.1325204956826843No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15370.13048302489704405No Hit
CCCTGGGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACTTGCACCCCGTAGTATCGCCGGGAAATGAAGAGCCC15150.1286153433435405No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15130.1284455541114038No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15070.12793618641499374No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14880.12632318870969522No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14850.12606850486149018No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACT14740.1251346640847384No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATTCTCAGTGTAAAATCTGTGCCTGATCGACTGCCA14630.12420082330798664No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14620.12411592869191827No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14520.12326698253123484No Hit
ACCTATTACAACCCGTCCCTCCAGAGTCGAGTCACCATATTCGTGGACAC14170.12029567096884283No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14050.1192769355760227No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13670.11605094016542564No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13610.11554157246901559No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13580.11528688862081056No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13570.11520199400474221No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13540.11494731015653718No Hit
GACTTGCACCCCGTAGTATCGCCGGGAAATGAAGAGCCCCGCACAATAAT13280.11274005013876025No Hit
GTAATAGGTGGTCCCACTACCAAAGATACTCCCAATCCACTCTAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC13130.11146663089773509No Hit
CAGTAATGGATATCACTATCTGGATTGGTACGTGCAGAAGCCAGGGCAGT13100.11121194704953007No Hit
CTGCTAATTCTCAGTGTAAAATCTGTGCCTGATCGACTGCCACTGAACCT12980.11019321165670994No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12820.10883489779961644No Hit
GGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTCGATCAGGCACAGATT12820.10883489779961644No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT12740.10815574087106969No Hit
CCGTAGTATCGCCGGGAAATGAAGAGCCCCGCACAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAA12560.10662763778183952No Hit
GGGCTAGAGTGGATTGGGAGTATCTTTGGTAGTGGGACCACCTATTACAA12500.10611827008542944No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12240.10391101006765252No Hit
CCACTGAACCTGTCAGGGACCCCGGAGGCCCGAGCAGAATCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGG12140.10306206390696909No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12130.10297716929090074No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12050.102298012362354No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12040.10221311774628564No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12010.10195843389808061No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12000.10187353928201227No Hit
GTGTATTATTGTGCGGGGCTCTTCATTTCCCGGCGATACTACGGGGTGCA11980.10170375004987559No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11910.10110948773739718No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11840.10051522542491877No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11820.10034543619278208No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG11800.10017564696064542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)