FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177931
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGATATCACTATCTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATT57430.48754978008049704No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48300.4100409956100994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34460.29254684697151195No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34120.28966043002518826No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGGGCTGGATCCG33200.2818501253469006No Hit
GTGTAAAATCTGTGCCTGATCGACTGCCACTGAACCTGTCAGGGACCCCG29710.25222190433904873No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGA28820.24466628350896616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28540.24228923425905252No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28090.23846897653597704No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCACGTCTAGTCAGAGCCTCCTG27560.23396956188435486No Hit
CTACAAACTCCTCGGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT27470.2332055103397398No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAGA27300.23176230186657795No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27120.23023419877734774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26620.22598946797393057No Hit
GATTTTACACTGAGAATTAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT26000.22072600177769328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25650.21775469021530122No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATTCTCAGTGTAAAATCTGTGCCTGATCGACTGCCA25420.21580211404572933No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25360.21529274634931928No Hit
GGCCAAAAGTGGCCCGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTG24210.20552986550145977No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT23630.20060597776949585No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22880.19423888156437008No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATTCTCAGTGTAAAAT22600.19186183231445644No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22470.19075820230556798No Hit
ATCCATTACTGTGCAGGAGGCTCTGACTAGACGTGCAGGAGATGGAGGCC22400.1901639399930896No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22250.18889052075206442No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21690.1841364222522372No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21180.17980679683275166No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21020.17844848297565816No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20770.1763261175739496No Hit
GTTCAGTGGCAGTCGATCAGGCACAGATTTTACACTGAGAATTAGCAGAG20440.17352459524369424No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20270.1720813867705324No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20260.17199649215446405No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19840.16843091827959364No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19760.16775176135104689No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG19170.1627429790030146No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19120.16231850592267288No Hit
GAATTAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAA18950.16087529744951104No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18920.16062061360130603No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGG18560.15756440742284564No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18520.1572248289585723No Hit
CTCCTGCACAGTAATGGATATCACTATCTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT18410.1562909881818205No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTGCTCGGGCC18220.15467799047652198No Hit
GTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCACGTCTAGTCAGAGCCTCCTGCA18190.15442330662831694No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18060.15331967661942847No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17920.15213115199447166No Hit
GTCCCTGACAGGTTCAGTGGCAGTCGATCAGGCACAGATTTTACACTGAG17480.14839578888746455No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17440.14805621042319117No Hit
GTATTATTGTGCGGGGCTCTTCATTTCCCGGCGATACTACGGGGTGCAAGTCTGGGGCCAAGGGACCACGGTCAC17320.14703747503037104No Hit
GGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTGCTCGGGCCTCCGGGGT16850.1430474280751589No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16490.13999122189669852No Hit
CGTCTAGTCAGAGCCTCCTGCACAGTAATGGATATCACTATCTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTC16370.13897248650387842No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGA15990.13574649109328135No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15850.13455796646832455No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCC15750.13370902030764112No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCGGGCCACTTTTGGCCA15710.13336944184336774No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCC15630.132690284914821No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15170.1287851325756772No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14690.12471019100439669No Hit
GTCAGGGACCCCGGAGGCCCGAGCAGAATCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCACGTA14500.12309719329909816No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14300.12139930097773129No Hit
GGATATCACTATCTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTGCT14160.1202107763527745No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14090.11961651404029608No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13960.11851288403140761No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACT13930.11825820018320259No Hit
CCCTGGGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACTTGCACCCCGTAGTATCGCCGGGAAATGAAGAGCCC13890.1179186217189292No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13840.11749414863858748No Hit
ATTACAACCCGTCCCTCCAGAGTCGAGTCACCATATTCGTGGACACGTCCAAGAGCCAGTTCTCCCTGAAGTTGA13820.1173243594064508No Hit
CTGCTAATTCTCAGTGTAAAATCTGTGCCTGATCGACTGCCACTGAACCT13790.11706967555824577No Hit
GGGCTAGAGTGGATTGGGAGTATCTTTGGTAGTGGGACCACCTATTACAA13560.11511709938867387No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTAATTCTCAGTGTAAAATCTGTGCCTGATCGACTGCCA13550.11503220477260552No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13540.11494731015653718No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13510.11469262630833216No Hit
GGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTCGATCAGGCACAGATT13460.11426815322799044No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13300.11290983937089694No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13260.11257026090662357No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGGGCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13180.1118911039780768No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12840.10900468703175313No Hit
CTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCACGTCTA12750.10824063548713805No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12690.10773126779072797No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12690.10773126779072797No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12670.10756147855859129No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12640.10730679471038626No Hit
GTAATAGGTGGTCCCACTACCAAAGATACTCCCAATCCACTCTAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC12600.10696721624611288No Hit
GACTTGCACCCCGTAGTATCGCCGGGAAATGAAGAGCCCCGCACAATAAT12510.10620316470149781No Hit
ACCTATTACAACCCGTCCCTCCAGAGTCGAGTCACCATATTCGTGGACAC12010.10195843389808061No Hit
CCGTAGTATCGCCGGGAAATGAAGAGCCCCGCACAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAA11810.10026054157671374No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG11800.10017564696064542No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11800.10017564696064542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTG150.002480873468.1038910
GGTATCA10550.047.7837521
ATACGTA1000.044.2656511
GTGGTAT3150.042.1720851
TGGTATC3100.040.6460952
GAATACG1100.040.2432069
TACGTAC1100.040.24149712
ATGGCAA2050.038.7119370
CGGTACC459.626095E-538.59246445
GTATCAA26300.037.6884461
GTGCTAG1300.036.6822241
AATACGT1250.035.4140210
CTAGAGA1350.035.3116264
TACATAT1300.034.1272926
GATTCAC1600.034.062071
TATCAAC30300.032.144172
TCAAATG1407.2759576E-1231.2632866
TTAACGG553.339147E-431.23077835
TAAGGTT1550.031.16142839
ATCAACG31450.031.0717893
Produced by FastQC (version 0.11.5)