FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215854
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGCAGATACA26470.2177070602226912No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26060.21433494482067747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25250.20767296073377234No Hit
GTGTATTACTGTGCGTCCTATAGCAGCAACTGGGTCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC20480.16844127666644185No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19250.15832493046040066No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19150.15750246328917783No Hit
CAGTAATACACAGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGCAGATA18930.15569303551248753No Hit
GTTTATTACTGCATACAAGCTACACAATTTCCTCTGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18250.15010025874817207No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16440.13521360294903828No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16410.13496686279767142No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15800.129949813053212No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA15520.12764690497378797No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT15370.12641320421695368No Hit
GTATGATGGAAGTCTAAAATTCTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAGGAA14950.12295884209781766No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14620.12024470043278224No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14580.11991571356429309No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14510.11933998654443709No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14370.11818853250472508No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14070.11572113099105649No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13840.11382945649724391No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13730.11292474260889876No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG13560.1115265484178199No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGCCAAAGCCTCGTA13550.11144430170069762No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13040.10724971912746102No Hit
GTATTACTGTGCGTCCTATAGCAGCAACTGGGTCTTTGACTACTGGGGCC12650.10404209715969186No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12470.10256165625149073No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12280.1009989686261673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1700.048.6876740
CGTATGC1800.046.06342741
ACATGCG1850.044.23492427
ATGCCGT2000.041.6658144
GGTATCA12500.040.02311
GTGGTAT4000.039.9890671
GTATCAA36850.038.5125241
ATCTCCG2300.038.4723710
TGGTATC4100.038.1836472
ACGAGAC2250.037.83183722
TGCGCAG2300.037.1285630
CCCACGA2150.036.41065619
CGAGACA2400.035.47610523
ATGCGCA2450.034.83381329
TATCAAC41200.034.363672
CATGCGC2500.034.10478228
ATCAACG42550.033.3533823
GTATGCC2700.033.31949642
TCAACGC42900.033.001944
CAACGCA43250.032.734875
Produced by FastQC (version 0.11.5)