FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215854
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGCAGATACA23260.1913058640264374No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22680.1865355544333448No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20970.17247136580543387No Hit
GTGTATTACTGTGCGTCCTATAGCAGCAACTGGGTCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC20080.16515140798155042No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19380.15939413778299039No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19300.1587361640460121No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18540.15248541354471837No Hit
GTTTATTACTGCATACAAGCTACACAATTTCCTCTGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17410.14319153450990002No Hit
CAGTAATACACAGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGCAGATA16800.13817448476544059No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15820.13011430648745656No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15340.12616646406558682No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA15030.12361681583479595No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14330.11785954563623594No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14240.11711932518213536No Hit
GTATGATGGAAGTCTAAAATTCTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAGGAA14110.11605011785954564No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13640.1121845221547982No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13600.11185553528630905No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13530.11127980826645305No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13460.11070408124659704No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT13270.1091413936212736No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG12690.104371084028181No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12570.1033841234227136No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12380.10182143579739016No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12220.10050548832343358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4250.040.7666471
GTATCAA36250.038.6111681
GGTATCA13350.037.1531221
CTAACGC558.280176E-637.0209473
TGGTATC4750.035.021942
CGCGTTT400.00272364634.16494831
TATCAAC40600.033.7825932
ATCAACG41500.033.2816853
TCAACGC42050.032.839624
CAACGCA42850.032.066835
AACGCAG43600.031.9042956
CCTAACG652.5912312E-531.3383122
AGAGTAC45150.030.88289611
ATAAACG553.5876772E-430.850793
CGCAGAG45850.030.5606488
CAGAGTA45900.030.3043610
GCATAGG450.00500091330.1776312
ACGCAGA46250.030.1496227
GCAGAGT46900.029.8041119
CGGGAAT4250.027.95868717
Produced by FastQC (version 0.11.5)