FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929039
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77930.4039835379170665No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC69620.3609050931577848No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54700.28356088186915873No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT54430.28216122120910986No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG52660.2729856679932339No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG50930.2640174719121801No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTCCGCCGAATATGAA46140.23918645501723917No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT43480.22539720555157255No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41540.21534038451270296No Hit
GACCTAGGACGGTCACCCTGGTCCCTCCGCCGAATATGAAATTGTGGTTGCCCGCATATGAGCCCGCATATGAGC41140.21326681316448243No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39430.20440229565083962No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG39270.2035728671115514No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37280.1932568496541542No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37020.19190902827781087No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC36920.19139063544075574No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG36690.19019833191552893No Hit
GTAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA36550.18947258194365174No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTC36240.1878655641487808No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT35810.18563647494944374No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32830.17018836840520074No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATGACTCTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC32680.16941077914961802No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30650.15888740455739878No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30410.15764326174846646No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29970.15536233326542387No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29730.15411819045649156No Hit
GATTATTACTGCGGCTCATATGCGGGCTCATATGCGGGCAACCACAATTT29620.1535479583357309No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28600.14826035139776853No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27750.14385401228279987No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27650.14333561944574474No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATGACTCTGTCTCCTGGTACCAACAGCA27420.14214331592051793No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27340.1417286016508738No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC26450.13711490540108312No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26350.136596512564028No Hit
GTGTATTACTGTGCAAGAGGACATATGCGTCGGGGCTTTGACTCTTGGGGCCAGGGAAGCCTGGTCACCGTCTC26100.13530053047139018No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC25900.1342637447972799No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25660.13301960198834756No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC25620.1328122448535255No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24880.12897613785931752No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24620.12762831648297418No Hit
GGATGAGGCTGATTATTACTGCGGCTCATATGCGGGCTCATATGCGGGCA24360.1262804951066308No Hit
GATTATTACTGCGGCTCATATGCGGGCTCATATGCGGGCAACCACAATTTCATATTCGGCGGAGGGACCAGGGTG24140.1251400308651095No Hit
CCCCTGAGGGCCGCTTATCGACCTCAGAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA23900.1238958880561772No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCGGCTCATAT23770.12322197736800553No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23430.12145944172201806No Hit
CCCTGGTCCCTCCGCCGAATATGAAATTGTGGTTGCCCGCATATGAGCCCGCATATGAGCCGCAGTAATAATCAG23120.11985242392714714No Hit
GCTTATGACTCTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTCTGAGGTCGATAAG22660.11746781687669353No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG21710.11254308492466975No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21220.11000296002309957No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT21160.1096919243208665No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21140.10958824575345548No Hit
GGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG21010.10891433506528381No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTG20750.10756651368894045No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20510.10632237088000812No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTC20410.105803978042953No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC20410.105803978042953No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT20390.10570029947554198No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20360.10554478162442542No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20300.10523374592219235No Hit
GGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAG19430.10072372823981268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)