FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630574
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGCACCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT140450.8613531185950469No Hit
CTCCTAAGCTGCTCGTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT97070.5953118349734511No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT86870.5327571763072391No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC86760.5320825672431917No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC72900.4470818251732212No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAGTTCTTA72450.4443220608203001No Hit
CTTTTATACAACTCCAACAATAAGAACTATTTAGCTTGGTACCAGCAGAA65880.4040295012676518No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC62540.3835459169593039No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC55610.3410455459243187No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG54590.3347900800576975No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49580.3040647035951757No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45570.27947213680581195No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43090.26426276881638No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA42330.2596018334647799No Hit
GTTTATTACTGTCAGCAGTATTATAGCACCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAA41800.2563514443380061No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40560.24874676034329016No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT39980.24518973073285852No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39290.24095809205837943No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC39230.24059012347798997No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39210.2404674672845268No Hit
GTGCTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT37140.22777255126108964No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36830.2258713802624107No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36210.22206903826505267No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATCTTTTATA36060.22114911681407898No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAACGTC35980.22065849204022633No Hit
AACCAGGACAGCCTCCTAAGCTGCTCGTTTACTGGGCATCTACTCGGGAA35820.21967724249252105No Hit
GTATAAAAGATTGTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA35550.2180213838807684No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35110.21532294762457885No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA34570.2120112304010735No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34280.21023271559585766No Hit
TTATAGCACCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT32230.19766045576588365No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATCTTTTATACAACTCCAA31680.19428741044564674No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30930.18968780319077821No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30490.18698936693458867No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG30410.18649874216073603No Hit
GTCAGGGACCCCGGATTCCCGAGTAGATGCCCAGTAAACGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA29690.18208311919606224No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29630.18171515061567275No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC29560.1812858539385517No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT29530.18110186964835695No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29100.178464761488899No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28780.17650226239348843No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGCACC28610.17545968474905158No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC28240.17319054516998308No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATCTTT27880.1709827336876462No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAATAGTTCTTATTGTTGGAGTTG27710.16994015604320933No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25970.15926906721191433No Hit
CTATTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCGTTTACTGGGCATCTACTCGGGAATC24860.15246164847470892No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24310.14908860315447198No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23990.14712610405906143No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGCACCCCGTGGACGTT23770.14577688593096666No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23740.1455929016407719No Hit
GGCAGGGGCAGCAAGATGGTGTCGCAGACCCAGGTCTTCATTTCTCTGTT23620.14485696447999294No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23240.14252649680419288No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23000.14105462248263495No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22980.1409319662891718No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG22580.13847884241990857No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22530.13817220193625068No Hit
CCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC22360.1371296242918138No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22300.13676165571142432No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22250.13645501522776643No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22060.1352897813898664No Hit
GCTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22020.13504446900294007No Hit
ATAGCACCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGA22000.1349218128094769No Hit
CTACGGGGACACCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG21990.13486048471274534No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21910.1343698599388927No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21730.13326595419772422No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21660.13283665752060317No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21540.1321007203598242No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCC21450.13154876748924No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC20880.1280530659755399No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20560.12609056688012932No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGCACCCCGT20320.1246186925585714No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20040.12290150585008715No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC19930.12222689678603976No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19850.12173627201218712No Hit
GAACTATTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGC19730.12100033485140815No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19660.12057103817428709No Hit
GGATCTCTGGTGCCTACGGGGACACCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG19580.12008041340043445No Hit
CAGCAAGATGGTGTCGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA19440.11922182004619232No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19360.11873119527233969No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19360.11873119527233969No Hit
GTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC19250.1180565862082923No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19190.11768861762790281No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19130.11732064904751334No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19100.11713666475731858No Hit
GCAAGATGGTGTCGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC18890.11584877472595541No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18730.11486752517825011No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18570.11388627563054482No Hit
ATTATAGCACCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAA18370.11265971369591322No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18330.11241440130898689No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18010.11045190221357633No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG17870.1095933088593342No Hit
GCAGAAACCAGGACAGCCTCCTAAGCTGCTCGTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCTGACCGATT17760.10891869979528682No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAGT17590.10787612215084993No Hit
GGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC17590.10787612215084993No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTCGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT17570.10775346595738677No Hit
GCTAAATAGTTCTTATTGTTGGAGTTGTATAAAAGATTGTGGCTGGACTT17560.10769213786065521No Hit
ATTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCGTT17510.1073854973769973No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17460.10707885689333939No Hit
ACCATCAACTGCAAGTCCAGCCACAATCTTTTATACAACTCCAACAATAA17410.1067722164096815No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17370.10652690402275518No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAACGTCCACGGGGTG17170.10530034208812357No Hit
CTGCAAGTCCAGCCACAATCTTTTATACAACTCCAACAATAAGAACTATT17150.10517768589466041No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17140.10511635779792883No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17120.10499370160446567No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17020.10438042063714986No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16710.10247924963847088No Hit
GTAAACGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA16640.1020499529613498No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16590.10174331247769192No Hit
TTATTACTGTCAGCAGTATTATAGCACCCCGTGGACGTTCGGCCAAGGGA16420.10070073483325503No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCGTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCTGA16410.10063940673652345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT601.1350494E-768.96586670
ACGCGAA200.00643426653.5384458
TATCTCG1400.039.07472236
ACCGAAG350.001477010738.7020317
ATCTCGT1450.037.774137
GGTACGT755.251313E-737.332468
GTACGTA855.7804755E-736.8770669
GACAGGT1750.036.772571
GGTATCA14850.034.667741
TCGTATG1700.034.404540
TGGTACG801.3106528E-633.2136867
GACGGAC2150.033.12753326
GATTGTG35050.033.05639670
GTATCAA33050.032.8959161
ATGCCGT1800.032.7143344
CGTATGC1900.032.6527541
ACGAGAC2200.032.34185422
GTGGTAT6600.031.8167761
TCCTATC2050.031.59862933
TCTTGAG7900.031.42748370
Produced by FastQC (version 0.11.5)