FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630574
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGCACCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT132070.8099601735339825No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC86920.5330638167908969No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82800.5077966409374859No Hit
CTCCTAAGCTGCTCGTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT81000.4967575835258013No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAGTTCTTA65940.4043974698480412No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC62000.3802341997357986No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61120.3748373272234195No Hit
CTTTTATACAACTCCAACAATAAGAACTATTTAGCTTGGTACCAGCAGAA53550.32841195799761314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51420.3153490733937865No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49300.3023475168866914No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49050.3008143144684019No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47290.2900205694436438No Hit
GTGCTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT41620.2552475385968377No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40180.2464162926674901No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39320.24114207634857418No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38740.23758504673814254No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAACGTC38580.23660379719043723No Hit
GTTTATTACTGTCAGCAGTATTATAGCACCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAA38240.23451864190156352No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA37960.23280145519307927No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36640.22470614642451062No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34820.213544432819363No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34720.2129311518520472No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34460.21133662133702608No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34280.21023271559585766No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC34130.20931279414488396No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT32860.20152412585997323No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATCTTTTATA31980.19612725334759415No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31970.1960659252508626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31480.19306084851101513No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAGT31450.1928768642208204No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31170.19115967751233615No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30220.185333508322836No Hit
AACCAGGACAGCCTCCTAAGCTGCTCGTTTACTGGGCATCTACTCGGGAA29440.18054991677777274No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28450.17447843520134626No Hit
GTATAAAAGATTGTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA28250.17325187326671468No Hit
TTATAGCACCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT28110.17239327991247255No Hit
GTCAGGGACCCCGGATTCCCGAGTAGATGCCCAGTAAACGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA28010.17177999894515675No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28000.17171867084842515No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27260.1671803916902882No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC26850.16466593972429341No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26770.16417531495044077No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATCTTTTATACAACTCCAA26200.1606796134367407No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGCACC26020.15957570769557222No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAATAGTTCTTATTGTTGGAGTTG25190.15448547566685106No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25110.15399485089299841No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAGTAGATGCCCAGTAAA25040.15356555421587736No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24130.14798469741330356No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24090.1477393850263772No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24010.1472487602525246No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC23920.14669680738194035No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23450.14381438683555606No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC23330.1430784496747771No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATCTTT23320.14301712157804553No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22740.13946009196761386No Hit
GCTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22390.13731360858200856No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22000.1349218128094769No Hit
GGGTGCTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT21940.13455384422908745No Hit
GGCAGGGGCAGCAAGATGGTGTCGCAGACCCAGGTCTTCATTTCTCTGTT21850.13400189135850318No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG21560.13222337655328736No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21480.13173275177943472No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGCACCCCGT21360.13099681461865575No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAACGTCCACGGGGTG21360.13099681461865575No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20940.1284210345559294No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGCACCCCGTGGACGTT20910.12823705026573465No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20570.1261518949768609No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20520.125845254493203No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20480.1255999421062767No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20450.12541595781608195No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC20390.12504798923569246No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20210.12394408349452402No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20100.12326947443047663No Hit
CCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC19880.12192025630238185No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19680.12069369436775025No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19530.11977377291677654No Hit
CTATTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCGTTTACTGGGCATCTACTCGGGAATC19490.11952846052985024No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19440.11922182004619232No Hit
ATAGCACCCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGA19250.1180565862082923No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18960.11627807140307646No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18890.11584877472595541No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18620.11419291611420274No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18600.11407025992073956No Hit
GCAAGATGGTGTCGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC18410.11290502608283955No Hit
CTACGGGGACACCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG18360.11259838559918164No Hit
GGATCTCTGGTGCCTACGGGGACACCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18220.1117397922449395No Hit
CAGCAAGATGGTGTCGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA18090.11094252698742896No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17990.11032924602011317No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17530.10750815357046047No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17280.10597495115217095No Hit
GAACTATTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGC17230.10566831066851304No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17220.10560698257178146No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16950.10395112396002881No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTCGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT16930.10382846776656564No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16880.10352182728290774No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16560.10155932818749716No Hit
GGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16380.10045542244632871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA307.0090604E-445.0326613
ACCCGTT404.953846E-543.1970742
GAACGCT350.001084979541.20768461
CCGTTTT350.001316050339.62127344
GGTATCA16900.036.2182241
GACAGGT2000.033.8170131
GTATCAA35950.032.5471151
ACGGGTC1307.2759576E-1231.20133416
ATGGACG553.4373286E-431.07767936
CTAACGC553.686556E-430.7087963
CGCTTAA450.004998699330.18096529
CGGGTAT450.005128117330.0236136
GATTGTG32550.029.98832570
TAGGACC704.4501357E-528.952234
TATCAAC41600.027.864812
TCAACGC41750.027.8311254
ATCAACG41650.027.8185563
AACGCAG42150.027.64636
CAACGCA42500.027.4186255
TGTTAGA27000.027.3152970
Produced by FastQC (version 0.11.5)