FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444993
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19730.4433777609984876No Hit
GCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAATAAATT16400.368545123181713No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14530.3265219902335542No Hit
GACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAATAAATTATTGTTGCCTGCATATGAGCTGCAGTAATAATCAG13640.3065216756218637No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG13250.2977574928144937No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13020.29258887218450624No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12200.2741616160254206No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTC12100.27191438966455656No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11260.25303768823329803No Hit
GATTATTACTGCAGCTCATATGCAGGCAACAATAATTTATTCGGCGGAGGGACCAAGCTGAGCGTCCTAGGTCAG10800.2427004469733232No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9910.22270013236163266No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT9850.22135179654511422No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC9030.20292454038602853No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC8990.2020256498416829No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8640.19416035757865854No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8590.1930367443982265No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8300.18651978795172058No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCAGAGACGGTCAGGGAGG8260.18562089740737495No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8190.18404783895477006No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8110.1822500578660788No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG7900.17753088250826418No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7760.17438476560305444No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7630.17146337133393108No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7570.1701150355174126No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATGACTATGTCTCCTGGTACCAACAACA7400.16629475070394367No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7350.16517113752351162No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7330.16472169225133879No Hit
CCCCTGGGGGCCGCTGAGTGACCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA7310.16427224697916598No Hit
CCCCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGT7300.16404752434307954No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7280.16359807907090673No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7240.1626991885265611No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7220.16224974325438826No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6860.1541597283552775No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGC6650.14944055299746287No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC6600.1483169398170308No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG6540.14696860400051237No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6500.14606971345616673No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6350.14269887391487057No Hit
TCCTCAGACTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC6240.14022692491792005No Hit
GTCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACAATAATTTATTCGGCGGAGGGACCAAG6200.1393280343735744No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT6180.13887858910140158No Hit
GCTTGGTCCCTCCGCCGAATAAATTATTGTTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGA6120.13753025328488314No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6060.13618191746836467No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6050.13595719483227828No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT6020.13528302692401903No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTCATAA5850.13146274211055006No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5840.13123801947446365No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT5780.12988968365794518No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT5610.1260693988444762No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5530.12427161775578494No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT5500.12359744984752569No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTG5440.12224911403100724No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5410.121574946122748No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC5380.12090077821448877No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC5370.12067605557840234No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA5350.12022661030622953No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT5340.12000188767014312No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAATAAATTATTGTTGC5330.11977716503405672No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT5310.1193277197618839No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC5300.11910299712579747No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5250.11797938394536543No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT5180.11640632549276056No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC5170.11618160285667414No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5120.11505798967624209No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC5100.11460854440406927No Hit
GCTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCACTCAG5060.11370965385972365No Hit
GGACCAAGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG5010.11258604067929158No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC4990.11213659540711876No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC4940.11101298222668671No Hit
CCCTGACCGTCTCTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGC4640.1042713031440944No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4590.10314768996366236No Hit
CTCCCTGACCGTCTCTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACT4530.10179935414714389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)