FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444993
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17820.4004557375059833No Hit
GCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAATAAATT15120.33978062576265244No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15000.3370839541296155No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13530.3040497266249132No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13360.30022944181144423No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG13010.2923641495484199No Hit
GACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAATAAATTATTGTTGCCTGCATATGAGCTGCAGTAATAATCAG11690.2627007615850137No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11580.2602288125880632No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTC11140.2503410166002611No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10710.2406779432485455No Hit
GATTATTACTGCAGCTCATATGCAGGCAACAATAATTTATTCGGCGGAGGGACCAAGCTGAGCGTCCTAGGTCAG10610.23843071688768136No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC9660.2170820664594724No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9650.21685734382338598No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9100.20449759883863342No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8980.20180092720559648No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC8980.20180092720559648No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT8890.19977842348081878No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8710.1957334160312634No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8480.19056479540127597No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8250.18539617477128853No Hit
CCCCTGGGGGCCGCTGAGTGACCTCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA7990.17955338623304187No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7690.17281170715044955No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7530.169216144973067No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7270.16337335643482032No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATGACTATGTCTCCTGGTACCAACAACA7260.16314863379873393No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7200.16180029798221546No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7150.1606766848017834No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7090.15932834898526493No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7020.15775529053266008No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCAGAGACGGTCAGGGAGG6970.15663167735222802No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6800.15281139253875903No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6710.15078888881398134No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG6700.15056416617789495No Hit
CCCCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGT6370.1431483191870434No Hit
GTCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAACAATAATTTATTCGGCGGAGGGACCAAG6290.1413505380983521No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6140.13797969855705597No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT6120.13753025328488314No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT6100.1370808080127103No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6060.13618191746836467No Hit
GCTTGGTCCCTCCGCCGAATAAATTATTGTTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGA6000.1348335816518462No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5870.13191218738272287No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT5760.12944023838577237No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTCATAA5700.1280919025692539No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGC5680.12764245729708107No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT5660.12719301202490824No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5560.12494578566404416No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5550.12472106302795774No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG5550.12472106302795774No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC5450.12247383666709365No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5440.12224911403100724No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT5440.12224911403100724No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5340.12000188767014312No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC5320.1195524423979703No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC5310.1193277197618839No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5300.11910299712579747No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC5250.11797938394536543No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA5150.11573215758450134No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5140.11550743494841492No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5120.11505798967624209No Hit
GGACCAAGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG5090.11438382176798287No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC4970.11168715013494596No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT4900.11011409168234106No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTG4890.10988936904625465No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC4820.1083163105936498No Hit
CCCTGACCGTCTCTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGC4660.10472074841626723No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAATAAATTATTGTTGC4580.10292296732757594No Hit
GCTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCACTCAG4570.10269824469148954No Hit
CTCCCTGACCGTCTCTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACT4490.10090046360279824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)