FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308002
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA76130.5820327491853988No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT45810.3502288222800882No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42380.32400562078651257No Hit
CTATAGGAGACTAGACTTTTCGCACAGTGATATACGGCCGTGTCCTCGAC40490.30955610159617497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37090.2835622575500649No Hit
GTATATCACTGTGCGAAAAGTCTAGTCTCCTATAGTGGGAGCCCCTTCGA35090.2682717610523531No Hit
CTCCTATAGTGGGAGCCCCTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAG34560.2642197794804595No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA32760.2504583326325189No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCACCTATGGCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG32230.24640635106062528No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31650.24197210707628888No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31210.2386081978467923No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC31140.23807303046937237No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30350.2320332843527762No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29920.2287458276057682No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29440.22507610844631734No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA26770.20466329562187213No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25550.19533609275826794No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATCATAATTGG25380.19403640055596247No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24470.1870792246495036No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT24240.18532081755226673No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC24160.18470919769235827No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23540.17996914377806764No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT23370.1786694515757621No Hit
CATCTATGGTGCATCCACCGGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA23270.17790492675087655No Hit
CCTATAGTGGGAGCCCCTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCC22960.17553489979373121No Hit
CCTTTAGCACCTATGGCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG22930.17530554234626552No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22700.17354713524902868No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC22690.17347068276654012No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22680.17339423028405154No Hit
GTCAGAGTGTGAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTG22310.1705654884319749No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22150.16934224871215794No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22070.16873062885224946No Hit
GGGCTGGAGTGGGTCTCATTTATTAGTGGTAGTGGTGGTAGAACATACTA20740.15856244868127115No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20660.15795082882136266No Hit
GTATCATAATTGGCCCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20640.15779792385638555No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGCTGCAGCTGTTGGAGT20590.15741566144394276No Hit
GTTTATTACTGTCAGCAGTATCATAATTGGCCCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20590.15741566144394276No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT20310.1552749919342631No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGGGGCTCCCA20230.15466337207435463No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG20000.15290496497711778No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19750.1509936529149038No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG19620.14999977064255254No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCACCTATGGCATGAGCTGGGTCCG19560.1495410557476212No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19490.14900588837020126No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT19440.1486236259577585No Hit
CCTATGGCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGG19200.14678876637803306No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCCGGT19130.14625359900061316No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19090.14594778907065892No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18880.14434228693839918No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC18790.14365421459600214No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC18630.14243097487618522No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGCCATAGGTGC18450.14105483019139115No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18310.1399844954365513No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGGC18240.1394493280591314No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17990.13753801599691745No Hit
GGGTTCCCTGGCCCCAGGGGTCGAAGGGGCTCCCACTATAGGAGACTAGA17840.13639122875958906No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA17820.13623832379461193No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT17780.1359325138646577No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17690.13524444152226067No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT17670.13509153655728356No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17660.13501508407479498No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17580.13440346421488653No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17510.13386829683746662No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17270.1320334372577412No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT17260.13195698477525264No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGAGTGTGAGCAG17090.13065729257294714No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG17080.13058084009045856No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17050.1303514826429929No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCAGAGTGTGAGCAGCAACTTAGCCTGGTA16920.12935760037064162No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16700.12767564575589335No Hit
GCCTGAGAGTCGAGGACACGGCCGTATATCACTGTGCGAAAAGTCTAGTCTCCTATAGTGGGAGCCCCTTCGACC16700.12767564575589335No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC16350.12499980886879379No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16350.12499980886879379No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT16300.12461754645635098No Hit
TTATGATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC16170.12362366418399971No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG15950.12194170956925142No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG15920.12171235212178573No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCACCTATGGC15910.12163589963929719No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA15660.11972458757708321No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG15510.11857780033975483No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA15460.11819553792731205No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15440.11804263296233493No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15310.11704875068998366No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGG15170.11597841593514384No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15060.11513743862776968No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14810.11322612656555571No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT14670.11215579181071589No Hit
GTATATCACTGTGCGAAAAGTCTAGTCTCCTATAGTGGGAGCCCCTTCGACCCCTGGGGCCAGGGAACCCTGGT14650.11200288684573877No Hit
GCCGTATATCACTGTGCGAAAAGTCTAGTCTCCTATAGTGGGAGCCCCTT14640.1119264343632502No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT14530.11108545705587607No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT14530.11108545705587607No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14460.11055028967845615No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA14070.10756864286140236No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG13590.10389892370195153No Hit
CTCCTGCTGGGCCAGTCAGAGTGTGAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCT13550.10359311377199729No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13480.10305794639457737No Hit
GGATACCAGTGGCCCCGGTGGATGCACCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGG13310.10175825419227187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA451.593749E-460.78546570
TAACCGG200.0076215551.29361714
TCTGGAG24400.048.7653970
TATACGC350.001407605139.0823487
GTATCAA19550.039.0297621
GACAGGT2150.035.012411
GGTATCA8300.034.628921
TTAACGG501.8945709E-434.39692735
AAACTCG400.002699039934.2284622
TGTGTAA501.9610122E-434.19574710
TGTTAGA9400.034.19182270
TATCAAC22350.033.8235862
ACTCGTA1500.032.92158559
ACGCAGG1052.6119128E-432.56364470
ACGGGCC959.562427E-932.40587616
ATCAACG23600.032.1696433
TCAACGC24100.031.5022244
AACGCAG24850.030.9631256
CAACGCA24650.030.9368865
CGCCAAC450.00448219430.86103846
Produced by FastQC (version 0.11.5)