FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709639
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAGCAATAAGAACTACTTAGCTTGGTACCAGCAGAA73770.43149460207681267No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT70230.41078847639764887No Hit
GTTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGCTGGAGCTGTATAAAACACT64810.3790858771939573No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAAGGAGTCTGGAGA56240.32895833564863697No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51230.2996539035433796No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48610.2843290308655804No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT43260.2530358748250362No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42890.25087167524840037No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37380.2186426491206623No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35410.2071197486720881No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35340.20671030550894076No Hit
GTTTATTACTGTCAGCAATATTATAACGTCGGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA32710.19132694095069191No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32290.18887028197180808No Hit
CTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAG31580.18471735845988538No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30600.17898515417582309No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30030.175651116990195No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29200.17079629091287693No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29140.17044533963017924No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29050.16991891270613269No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28880.16892455073848925No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA27840.16284139517172924No Hit
ATATTATAACGTCGGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT27380.16015076867104694No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26870.15716768276811655No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26100.15266380797349616No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25050.14652216052628655No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24710.14453343659099963No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC24360.1424862207752631No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23710.13868424854603809No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC23310.13634457332805347No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22860.13371243870782076No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22420.1311387959680377No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21630.1265179374125181No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTTTA21610.12640095365161885No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21410.12523111604262654No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCGTGGTTTCTGCTGGTA21230.12417826219453347No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20880.12213104637879693No Hit
GTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA20710.12113668441115348No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20470.11973287928036269No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20390.11926494423676577No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19490.11400067499630039No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18980.11101758909337No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18940.11078362157157154No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18940.11078362157157154No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC18850.11025719464752501No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18690.10932132456033115No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18470.10803450319043961No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18440.10785902754909078No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGTC18350.10733260062504424No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17880.10458348224391231No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTA17750.10382308779806732No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17480.1022438070259277No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17280.10107396941693537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8200.046.53993240
CGTATGC8300.046.1220241
ATCTCGT7150.045.7627537
GGTATCA14850.045.7312161
GTCGTAT605.9135346E-945.73115539
CCGTCTT8450.045.2081747
TGCCGTC8600.044.1746445
CGAGACT8850.043.51026523
GTATGCC8850.043.36683742
ATGCCGT8850.043.2060444
GACTAGG8900.042.94114726
AGACTAG9000.042.81768825
ACGAGAC9050.042.53999322
TAGGCAT9100.042.49782629
CTTATAC9150.042.4111481
CACGAGA9200.042.20287321
CTTGAAA9650.042.12697257
ACTAGGC9100.042.02941527
GAGACTA9200.041.8696924
GATCTCG8000.041.24415236
Produced by FastQC (version 0.11.5)