FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709639
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89830.525432562078895No Hit
GTTTTATACAGCTCCAGCAATAAGAACTACTTAGCTTGGTACCAGCAGAA61240.35820427587344467No Hit
GTTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGCTGGAGCTGTATAAAACACT59840.35001541261049846No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT55990.3274960386373965No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48430.2832761770174873No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAAGGAGTCTGGAGA43000.25151508593334615No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39510.2311014196564304No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37610.21998796237100346No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA34490.2017384956707235No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34220.20015921489858385No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33540.19618176702801No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32410.18957218453720348No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31960.18694004991697077No Hit
GTTTATTACTGTCAGCAATATTATAACGTCGGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA30740.1798040405021177No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29390.17190763664141961No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29180.17067930715197768No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28740.16810566441219463No Hit
CTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAG28290.16547352979196195No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26910.15740165028991498No Hit
ATATTATAACGTCGGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26030.15225436481034885No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25470.1489788195051704No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25260.14775049001572846No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC24780.14494287975414694No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24350.14242772889481345No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT23410.13692949213254962No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23350.13657854084985194No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG22520.13172371477253383No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22500.13160673101163461No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22330.13061236904399115No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22200.12985197459814615No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22060.12903308827185153No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22050.12897459639140194No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20240.11838756603002154No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGGCTGAAGATGTGGCAGTTTA20150.11786113910597501No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20030.11715923654057962No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCGTGGTTTCTGCTGGTA20010.11704225277968039No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC19940.11663280961653308No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19850.11610638269248653No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19570.11446861003989732No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA19520.11417615063764924No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19180.11218742670236231No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18680.10926283267988154No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18350.10733260062504424No Hit
TCTTCAGCCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC18230.10663069805964885No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17930.10487594164616039No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17910.10475895788526116No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17780.10399856343941616No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACCCGACGTT17740.10376459591761769No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17580.10282872583042386No Hit
GTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA17350.1014834125800827No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17290.10113246129738501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5300.042.881021
CGCAAAT450.001071789741.3096269
TGGTATC5450.041.05992
GGTATCA15650.040.5761951
GTATCAA36000.034.257521
GCGCAAA500.003263036532.92863568
ATTAGTA553.692072E-430.7010432
ATCAACG41950.029.6824933
TATCAAC42050.029.3883572
TCAACGC42700.029.0778564
CAACGCA42950.028.9086025
AACGCAG43450.028.8063876
GACAGGT3650.027.470111
ACGCAGA45700.027.3881287
CGCAGAG45350.027.305118
CGATTGC550.00845171327.10371263
AGAGTAC45900.026.97476811
CAGAGTA46600.026.64274210
CGATTAA550.00956957126.42197457
GCAGAGT46900.026.40279
Produced by FastQC (version 0.11.5)