FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925092
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGAACTTTTGGCCAGGGGACCAAACTGG27330.29543007614377814No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAAAGTGAAGTCTGTCCCAGACCCACTGCCA19920.21532993475243545No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19890.21500564268202516No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15070.16290271670277118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13840.14960674181594913No Hit
GTGTATTACTGTCAGCAGTATGGTAGAACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCA13750.14863386560471822No Hit
GTGTTAGCACCACCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT13360.14441806868938442No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13140.14203992683970892No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT12810.13847271406519568No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12620.13641886428593047No Hit
GTCTGGGACAGACTTCACTTTCACCATCAGCAGACTGGAGCCTGAAGATT11520.12452815503755303No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAAAGTGAAGTCTGTCCCAGACCCACTGCCA10870.11750182684532999No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA10680.11544797706606479No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10370.11209695900515841No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10160.10982691451228634No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT9820.10615160438096967No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9750.10539492288334565No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9540.1031248783904736No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9420.10182771010883243No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9410.10171961275202898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGC200.0079604650.7312
CTCGCGT200.00796386250.72551713
CGTATGC2250.049.1635541
CCTAACG508.119787E-847.348932
CTAACGC508.119787E-847.348933
TCGTATG2450.046.46615640
ACGAGAC2800.042.31014322
ATGCCGT2700.039.9520144
GTATGCC2700.039.78164742
CACGAGA3100.039.2947321
AATCTCG2450.039.14309336
ATCTCCG3200.039.10725810
CGAGACA3150.037.60901323
GGTATCA14050.037.3110541
CCCACGA3200.035.94611719
GCCCACG3300.035.8781618
ATCTCGT2600.035.63189337
TATACAC3950.035.1049963
CCGTCTT3100.034.96610347
TAGTGAT3450.034.8038570
Produced by FastQC (version 0.11.5)