FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925092
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28770.31099609552347224No Hit
GTATTACTGTCAGCAGTATGGTAGAACTTTTGGCCAGGGGACCAAACTGG25170.2720810470742369No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19120.2066821462081609No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAAAGTGAAGTCTGTCCCAGACCCACTGCCA17750.1918728083260908No Hit
GTGTATTACTGTCAGCAGTATGGTAGAACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCA13340.14420187397577755No Hit
GTGTTAGCACCACCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT12680.1370674484267511No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12310.1330678462250241No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12110.13090589908895547No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11610.1255010312487839No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11260.1217176237606638No Hit
GTCTGGGACAGACTTCACTTTCACCATCAGCAGACTGGAGCCTGAAGATT11230.12139333169025351No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10910.11793421627254369No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAAAGTGAAGTCTGTCCCAGACCCACTGCCA10870.11750182684532999No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10820.11696134006131283No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA9400.10161151539522556No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGT200.00800458350.66010713
GTATCAA29950.033.8755651
GGTATCA13450.033.6933861
ACGATCA850.003152569533.15598770
ATCAACG34050.029.7675693
CAACGCA34600.029.3840895
TCAACGC34700.029.3009914
TATCAAC34650.029.2616062
AACGCAG35050.028.8140956
TGTTAGA6750.028.1825970
TAGGTTG606.1363995E-428.1475476
CCTAACG500.00855107227.0377252
TACGAAG500.00855107227.0377252
ACGCAGA37950.026.6122267
CGCAGAG38150.026.4727138
AGAGTAC37850.026.23492211
TAGTGAT4100.025.77675870
GATATAC1059.1397123E-725.766941
GCAGAGT38950.025.6688279
CAGAGTA40350.024.61079210
Produced by FastQC (version 0.11.5)