FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414589
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATTACTGCTGCTCATATGCAGGTAGTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG69290.48982425283951736No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC62490.4417537532102964No Hit
GTAATAAACAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG56970.4027318182171642No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA56130.396793697674731No Hit
CTCCTGGACAGTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTT48490.3427850775030769No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46680.3299898415723578No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAG46140.32617247836650787No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCTCCTG44350.31351862625822763No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42260.29874401681336415No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40310.28495909412557285No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC37690.26643781338607897No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36830.2603583090212069No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35990.2544201884787737No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34720.24544231575390452No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC33840.23922142756659356No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAT33030.23349538275781867No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32500.22974871146318826No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31940.22578996443489946No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG30920.2185793894905163No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29460.20825837045247772No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG29340.2074100675178444No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28150.19899773008273075No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27980.19779596759200024No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27730.19602866981151415No Hit
GTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC27660.19553382643297806No Hit
GTACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCC27640.19539244261053915No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27500.19440275585346697No Hit
GACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCT27280.19284753380663924No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27210.19235269042810313No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26590.18796979193249772No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC25300.17885053538518964No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24650.17425556115592586No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23930.169165743548126No Hit
TGTAAGGACTGTGTGTACAGTAATATGTGGCTGTGTCCGCAGGGTCCATG22870.16167240095886506No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22720.16061202229057345No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22680.16032925464569567No Hit
CCCCTGAGGGCCGCTTATTGTCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA22430.1585619568652096No Hit
CCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGCCTCGTCCTCA22240.1572188105520402No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGC22160.15665327526228467No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22080.15608773997252912No Hit
GCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAT21360.15099792236472925No Hit
GTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAATTTTGTCTCCTGGTACCAACAGCA21250.1502203113413154No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21240.15014961943009597No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21060.148877165028146No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAATTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC20810.14710986724765993No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20770.14682709960278215No Hit
GCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGA20490.14484772608863777No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG20310.1435752716866878No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20300.14350457977546835No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20250.14315112021937113No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGAC19920.14081828714912953No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19710.13933375701352124No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC19680.1391216812798629No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCG19460.13756645923303518No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19440.1374250754105963No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGA19390.1370716158544991No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT17940.1268212887276799No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17780.12569021814816883No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17570.12420568801256054No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17500.12371084463402444No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACA17030.12038832480671063No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC16550.1169951130681774No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16540.11692442115695796No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG16520.11678303733451909No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGCCTCGTCCTCAGC16470.11642957777842186No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGTTTATTACTGCTGCTCATAT16300.11522781528769134No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16130.1140260527969608No Hit
GTCCAGGAGACCCAGGCACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC15930.11261221457257196No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15820.11183460354915808No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15790.11162252781549978No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGACAATAAGCGGCCCTCAGG15740.11126906825940255No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTAC15730.11119837634818311No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC15580.11013799767989148No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT15430.10907761901159983No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15400.10886554327794151No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15340.10844139181062486No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15270.10794654843208876No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15080.10660340211891935No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15070.1065327102076999No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACAATAAGCGGCCCTCAGGGGTTTCTAATCG15020.10617925065160268No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14830.10483610433843328No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14830.10483610433843328No Hit
CCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGG14800.10462402860477496No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC14700.10391710949258053No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14660.10363434184770276No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14630.10342226611404443No Hit
CAATAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCT14570.10299811464672777No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14290.1010187411325834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT208.671247E-568.7468237
AGGGGGG1650.053.24500370
GGTATCA10900.047.614951
ACACCGC306.6455133E-445.5217446
ACTACGC2150.042.8963627
ATCTCGT1050.042.5575637
ACGAGAC2200.041.8977322
CGAGACT2300.040.07892623
TCGCGTA350.001364830239.32869338
TCGTATG1150.038.9859540
TCTCGTA1150.038.90120738
GACTACG2400.038.4157626
GTATCAA26150.038.388561
CGAGCCC2550.037.48584415
CGTATGC1400.036.9919241
AGACTAC2500.036.87782725
CACGAGA2500.035.50444821
TATACAC3000.035.279353
GGATGGG10050.034.9668770
CTTATAC2700.034.1449281
Produced by FastQC (version 0.11.5)