FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414589
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATTACTGCTGCTCATATGCAGGTAGTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG69280.48975356092829786No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC59650.42167725042397475No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA53520.37834310884645644No Hit
GTAATAAACAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG50670.35819591414891533No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49970.35324748036355436No Hit
CTCCTGGACAGTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTT46820.33097952832942995No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCTCCTG44180.3123168637674971No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAG41730.29499734551873374No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39890.2819900338543563No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36680.25929793035291526No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35460.25067351718414327No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35420.25039074953926543No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33650.23787828125342417No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33470.23660582685147416No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32880.23243500408952705No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32240.22791072177148272No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32210.22769864603782441No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAT31980.2260727320797772No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC30080.21264126894808316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30070.21257057703686372No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28760.20330993666711675No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28520.2016133307978501No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27560.19482690732078364No Hit
GTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC27410.193766528652492No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27010.19093885220371432No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26290.18584903459591443No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26280.18577834268469498No Hit
GTACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCC26250.18556626695103665No Hit
CCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGCCTCGTCCTCA26090.18443519637152558No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25400.17955745449738406No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC25130.1776487728944591No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24690.17453832880080364No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC24520.1733365663100731No Hit
GACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCT24090.1702968141276371No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23350.16506561269739833No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22790.16110686566910953No Hit
CCCCTGAGGGCCGCTTATTGTCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA21870.15460320983692083No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21690.15333075543497088No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21370.1510686142759487No Hit
GCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCAT21240.15014961943009597No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20980.14831162973839043No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAATTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC20960.14817024591595157No Hit
GTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAATTTTGTCTCCTGGTACCAACAGCA20720.14647364004668495No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20570.1454132613783933No Hit
GCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCGATCATCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGA20470.14470634226619886No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20210.14286835257449337No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20090.14202004963986006No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGAC20020.14152520626132395No Hit
TGTAAGGACTGTGTGTACAGTAATATGTGGCTGTGTCCGCAGGGTCCATG19790.13989929230327677No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19680.1391216812798629No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19480.13770784305547407No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGC19450.13749576732181573No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19240.13601123718620745No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG18950.1339611717608436No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGA18930.13381978793840474No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18770.13268871735889365No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGCCTGGGTCTCCTGGACAGTCG18660.13191110633547978No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT18180.12851789459694654No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG18020.12738682401743545No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17850.12618506152670492No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT17730.12533675859207163No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17660.12484191521353552No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC17100.12088316818524673No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC17030.12038832480671063No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGACTACCTGCATATGAGCAGCAGTAATAAACAGCCTCGTCCTCAGC16820.11890379467110236No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16720.11819687555890791No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16430.11614681013354408No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACTAC16270.115015739554033No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16150.11416743661939971No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACA16040.11338982559598583No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15730.11119837634818311No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGTTTATTACTGCTGCTCATAT15580.11013799767989148No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC15520.10971384621257481No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15510.10964315430135538No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15180.10731032123111378No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC15050.10639132638526103No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14940.10561371536184715No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14920.10547233153940826No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGACAATAAGCGGCCCTCAGGGGTTTCTAATCG14600.1032101903803861No Hit
GGCAAAGCCCCCAAACTCATGATTTATGAGGACAATAAGCGGCCCTCAGG14580.10306880655794723No Hit
GTCCTTACATGTCGACCTCGCGCCCGCCAAAAATGGGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCT14410.1018670440672167No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC14210.10045320584282785No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14150.1000290543755112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.040.1638071
GTGGTAT4000.040.1136051
GTATCAA29050.038.898751
TGGTATC4600.034.861662
CGACCAA400.002552467234.62060543
ATACGTA701.0642998E-634.11027511
GGATGGG10350.033.98959470
TATCAAC34050.032.967562
ATCAACG35000.032.165663
TCAACGC34750.032.0991484
AACGCAG35800.031.3493676
CAACGCA35800.031.2529755
GACAGGT2100.030.8878781
ACGCAGA37150.030.1183327
ACCGTCT6600.029.76752969
CGCAGAG37650.029.7173048
TCCGAGA6050.029.64381470
AGAGTAC37650.029.3538611
GCAGAGT38600.028.9859189
CAGAGTA38700.028.82185710
Produced by FastQC (version 0.11.5)