FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238062
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGTGCTGTATTTGGGTCGTGGCATTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT60460.488343879385685No Hit
CATCTACACCACGGACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC55490.4482004939978773No Hit
GTATTTGGGTCGTGGCATTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC51950.41960741869147106No Hit
GTGTAGATGAGAGTGCGTGGAGCCTGGCCTGGGGTCTGTTGATACCAGCTGGGGTAATTACTAGAAGAGACTGAG49940.4033723674581725No Hit
GTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG48580.3923874571709656No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAAATGCCACGACCCAAATACAGCACACAGTAATAAT40260.32518565306099373No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38180.3083852020335007No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36250.29279632199356737No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35790.28908083763171794No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAAATGCCACGACCCAAATACAGCACA34770.28084215491631276No Hit
GTAATATACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATCTGCAGATACA33800.27300732919676074No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCCGGAGGGACAGTCACACTCACTTGTGGTTTGAACTCTGG33700.27219961520505437No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC30960.2500682518322992No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28980.23407551479651262No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28910.23351011500231814No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT28050.22656377467364314No Hit
CCCAAATACAGCACACAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC27720.22389831850101205No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27090.2188097203532618No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26650.21525577878975366No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26640.21517500739058304No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26570.21460960759638856No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26210.21170183722624553No Hit
CTCTCATCTACACCACGGACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG25820.20855175265859063No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24470.19764761377055431No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG24280.19611295718631214No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24130.19490138619875258No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24000.19385135800953426No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23600.1906205020427087No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23400.1890050740592959No Hit
GGATGATGCTTCTCCTCGGACTCCTTGTTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT23120.18674347488251802No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22540.18205873373062093No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22460.1814125625372558No Hit
CCCCAGAAGAGCGAGTGTCCGTGGTGTAGATGAGAGTGCGTGGAGCCTGG22380.1807663913438907No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21760.17575856459531106No Hit
GTTGATACCAGCTGGGGTAATTACTAGAAGAGACTGAGCCAGAGTTCAAACCACAAGTGAGTGTGACTGTCCCTC21390.1727700228259974No Hit
ACCCAAATACAGCACACAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT21110.1705084236492195No Hit
CACTTGTGGTTTGAACTCTGGCTCAGTCTCTTCTAGTAATTACCCCAGCTGGTATCAACAGACCCCAGGCCAGG21090.17034688085087824No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20000.16154279834127855No Hit
GTATATTACTGTGCGAAAGATCAGGCGTATGCAATGGACGTCTGGGGCCT19850.16033122735371896No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19630.1585542565719649No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19420.15685805718938145No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19280.1557272576009925No Hit
CTCATCTACACCACGGACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG19170.1548387722101155No Hit
CAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC19030.15370797262172653No Hit
AGATGAGAGTGCGTGGAGCCTGGCCTGGGGTCTGTTGATACCAGCTGGGG19010.15354642982338526No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGTTTATGGATCAGGAGTGG18930.15290025863002016No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18720.1512040592474367No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAT18690.1509617450499248No Hit
CCCCAGGCCAGGCTCCACGCACTCTCATCTACACCACGGACACTCGCTCT18300.14781166048226987No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18240.14732703208724604No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT17200.13892680657349954No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17170.13868449237598762No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCCGGAGGGACAGTCACA16970.13706906439257482No Hit
TTATTACTGTGTGCTGTATTTGGGTCGTGGCATTTGGATGTTCGGCGGAG16930.1367459787958923No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16890.13642289319920972No Hit
GTGCTGTATTTGGGTCGTGGCATTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG16580.1339189798249199No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16050.12963809566887602No Hit
ATACAGCACACAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGA15990.1291534672738522No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15570.12576106850868535No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15450.12479181171863767No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15280.12341869793273681No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15050.12156095575181211No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCCGGAG15000.12115709875595891No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14920.12051092756259378No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAGTCTGATTATTACTGTGTGC14860.12002629916756996No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGTTTATGGATCAGGAGTGGATT14760.11921858517586358No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC14730.11897627097835164No Hit
GTAGATGAGAGTGCGTGGAGCCTGGCCTGGGGTCTGTTGATACCAGCTGG14580.11776469999079206No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14490.11703775739825631No Hit
GCTTGGTCCCTCCGCCGAACATCCAAATGCCACGACCCAAATACAGCACACAGTAATAATCAGACTCATCATCTG14350.11590695780986734No Hit
GGCCTGGGGTCTGTTGATACCAGCTGGGGTAATTACTAGAAGAGACTGAG14190.11461461542313714No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14040.11340304443557753No Hit
GGCCCAGGCAGATGATGAGTCTGATTATTACTGTGTGCTGTATTTGGGTC14030.1133222730364069No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13990.11299918743972436No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC13770.11122221665797029No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC13450.10863753188450982No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13330.10766827509446217No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG13300.10742596089695024No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC13240.1069413325019264No Hit
CTTCTAGTAATTACCCCAGCTGGTATCAACAGACCCCAGGCCAGGCTCCACGCACTCTCATCTACACCACGGACA13230.10686056110275576No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGTTTATGG13050.10540667591768425No Hit
CCATCACGGGGGCCCAGGCAGATGATGAGTCTGATTATTACTGTGTGCTG12920.10435664772846594No Hit
GGTATCAACAGACCCCAGGCCAGGCTCCACGCACTCTCATCTACACCACG12780.10322584814007699No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12730.1028219911442238No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12570.10152964875749357No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC12500.10096424896329909No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12500.10096424896329909No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12480.10080270616495782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)