FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238062
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGTGCTGTATTTGGGTCGTGGCATTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT55620.44925052218709566No Hit
CATCTACACCACGGACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC51130.4129841639594786No Hit
GTATTTGGGTCGTGGCATTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC48040.38802580161575106No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38710.3126660861895446No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCCGGAGGGACAGTCACACTCACTTGTGGTTTGAACTCTGG37570.30345814668409177No Hit
GTGTAGATGAGAGTGCGTGGAGCCTGGCCTGGGGTCTGTTGATACCAGCTGGGGTAATTACTAGAAGAGACTGAG37100.2996618909230717No Hit
GTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG35830.28940392322840053No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAAATGCCACGACCCAAATACAGCACACAGTAATAAT35020.2828614398955787No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33110.26743410265398665No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32780.26476864648135556No Hit
GTAATATACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATCTGCAGATACA29120.23520631438490158No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAAATGCCACGACCCAAATACAGCACA28710.23189468701890537No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27190.2196174343449682No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC27020.2182443205590673No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT26980.21792123496238475No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25650.20717863887268975No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25170.20330161171249905No Hit
CTCTCATCTACACCACGGACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG24870.20087846973737988No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24810.20039384134235605No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24730.19974767014899092No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24290.1961937285854828No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG24210.1955475573921177No Hit
GGATGATGCTTCTCCTCGGACTCCTTGTTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT23880.1928821012194866No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23650.19102435903856188No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23500.1898127880510023No Hit
CACTTGTGGTTTGAACTCTGGCTCAGTCTCTTCTAGTAATTACCCCAGCTGGTATCAACAGACCCCAGGCCAGG23060.18625884648749416No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22580.1823818193273035No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22090.17842402076794214No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21290.17196230883429103No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAT21210.1713161376409259No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20900.16881222426663609No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20720.16735833908156456No Hit
CCCAAATACAGCACACAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC20630.1666313964890288No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20550.1659852252956637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20130.16259282653049686No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGTTTATGGATCAGGAGTGG19730.15936197056367127No Hit
CTCATCTACACCACGGACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG19170.1548387722101155No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18980.15330411562587334No Hit
GGTATCAACAGACCCCAGGCCAGGCTCCACGCACTCTCATCTACACCACG18880.15249640163416694No Hit
GTTGATACCAGCTGGGGTAATTACTAGAAGAGACTGAGCCAGAGTTCAAACCACAAGTGAGTGTGACTGTCCCTC18820.1520117732391431No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18300.14781166048226987No Hit
GTATATTACTGTGCGAAAGATCAGGCGTATGCAATGGACGTCTGGGGCCT18250.14740780348641666No Hit
GTGCTGTATTTGGGTCGTGGCATTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG18090.14611546109968646No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18040.14571160410383324No Hit
ACCCAAATACAGCACACAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT17960.14506543291046814No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17850.14417694751959112No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT17740.14328846212871407No Hit
CCCCAGGCCAGGCTCCACGCACTCTCATCTACACCACGGACACTCGCTCT17730.14320769072954342No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17690.1428846051328609No Hit
AGATGAGAGTGCGTGGAGCCTGGCCTGGGGTCTGTTGATACCAGCTGGGG17280.13957297776686467No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCCGGAGGGACAGTCACA16690.13480746521579695No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16670.13464592241745565No Hit
CCCCAGAAGAGCGAGTGTCCGTGGTGTAGATGAGAGTGCGTGGAGCCTGG16410.13254586603901905No Hit
TTATTACTGTGTGCTGTATTTGGGTCGTGGCATTTGGATGTTCGGCGGAG16290.13157660924897138No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16170.13060735245892371No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC16160.13052658105975307No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15780.12745726789126877No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15340.12390332632776063No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15170.12253021254185978No Hit
CTTCTAGTAATTACCCCAGCTGGTATCAACAGACCCCAGGCCAGGCTCCACGCACTCTCATCTACACCACGGACA15140.12228789834434785No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14870.12010707056674061No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCCGGAG14520.11728007159576824No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14020.11324150163723626No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13910.11235301624635922No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13910.11235301624635922No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATC13790.11138375945631157No Hit
GGCCTGGGGTCTGTTGATACCAGCTGGGGTAATTACTAGAAGAGACTGAG13740.11097990246045836No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13670.11041450266626389No Hit
CAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC13560.10952601727538686No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGTTTATGGATCAGGAGTGGATT13490.10896061748119237No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAGTCTGATTATTACTGTGTGC13430.10847598908616854No Hit
GGCCCAGGCAGATGATGAGTCTGATTATTACTGTGTGCTGTATTTGGGTC13330.10766827509446217No Hit
ATACAGCACACAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGA13160.10629516130856127No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12990.10492204752266042No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12890.104114333530954No Hit
GTCACACTCACTTGTGGTTTGAACTCTGGCTCAGTCTCTTCTAGTAATTA12770.10314507674090635No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGTTTATGG12650.10217581995085867No Hit
GTGTCCCCCGGAGGGACAGTCACACTCACTTGTGGTTTGAACTCTGGCTCAGTCTCTTCTAGTAATTACCCCAG12560.10144887735832292No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12540.10128733455998164No Hit
CCATCACGGGGGCCCAGGCAGATGATGAGTCTGATTATTACTGTGTGCTG12440.10047962056827525No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG12420.10031807776993398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)