FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221315
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT31211.4102071707746877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.3524388315297201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.34520931703680274No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC6580.2973137835212254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.2828547545353907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.26749203623794143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.26568465761471205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.22050019203397872No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGT4620.20875223098298806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.19790795924361204No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC4350.19655242527619005No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4320.19519689130876805No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.19022660009488737No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT4150.18751553216004338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.1839007749135847No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.17802679438808935No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.17712310507647472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.1689899012719427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.16537514402548403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3520.15904931884418136No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.1445902898583467No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.14278291123511738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.13510155208639268No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG2900.1310349501841267No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.12922757156089737No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.12877572690509004No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGG2840.1283238822492827No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.12696834828186068No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGG2800.12651650362605335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.12651650362605335No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT2780.1256128143144387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.12425728034701669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.12425728034701669No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTC2750.12425728034701669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.12335359103540203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.1229017463795947No Hit
CCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC2630.11883514447732871No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT2560.11567223188667737No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2510.1134130086076407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.11296116395183337No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATACC2470.11160562998441136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.10889456204956735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.1066353387705307No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.10302058152407202No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.1012132029008427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGG201.5754461E-4136.128270
ACGACTG151.9422617E-4129.1970569
TGTACTG100.0099978065129.1970469
CACGACT152.7903033E-4117.9651368
CCTTTAG153.9888948E-4107.8380865
CTCGTGG251.7947481E-594.372168
GATATAA208.759303E-488.4738568
CGCCACT150.001184870782.0113149
TTCCCCA150.001528728576.9151245
CGTGTAA200.001650975.4289561
GCCTCGT306.168935E-573.5806966
TGAGTGC150.002912774465.3808336
CACTTGT200.00300055964.8848452
AAGGGGG4900.064.59852669
TCGTGGC401.1745626E-464.5985269
AAAGGGG4700.061.4924668
AGGGGGG7300.060.91582570
CCACTTG356.619306E-660.65762751
CGTCATC200.00399676760.35751348
ATGTCTA250.00399868160.34316361
Produced by FastQC (version 0.11.5)