FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221315
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65042.9387976413708965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.3754829089758941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.36373494792490346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.31990601631159205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.2683957255495561No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.22998892980593272No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGT5030.22727786187108873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4890.22095203668978605No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.2200483473781714No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.2146262115084834No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4680.21146329891783203No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.19655242527619005No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT4280.1933895126855387No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC4200.18977475543908004No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.18435261956939203No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.1784786390438967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.1703454352393647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.16582698868129137No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.1576937848767594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.1540790276303007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.15317533831868604No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.14820504710480537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.1459458238257687No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGG3100.14007184330027336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3010.13600524139800735No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT2990.13510155208639268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.13419786277477805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.12877572690509004No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2840.1283238822492827No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTC2830.12787203759347537No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT2790.12606465897024602No Hit
CCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC2790.12606465897024602No Hit
GTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTA2720.1229017463795947No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGG2610.11793145516571402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.11702776585409937No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.1147685425750627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2480.11205747464021869No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT2470.11160562998441136No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.10979825136118203No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2430.10979825136118203No Hit
GGTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT2420.10934640670537468No Hit
GAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTT2380.10753902808214535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.10708718342633804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2350.10618349411472335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.10076135824503536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.10030951358922802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)