FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857988
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAG38810.4523373287272083No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAG33070.3854366261532795No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32170.3749469689552768No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGC31720.3697021403562754No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA28170.3283262702974867No Hit
GATTATTACTGTGAGACCTGGGACAGGAACACCCTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG27480.3202841997790179No Hit
CTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGTACT27230.317370406112906No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAGGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAG26710.3113097152873933No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26150.30478281747530267No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25110.2926614358242773No Hit
GCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGT24690.2877662624652093No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24250.2826379856128524No Hit
GAGTACAGGTGAGCTTGACCGAGGATCCCAGGGAAGCAGAGGCAGAGGAT22910.2670200515624927No Hit
GGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCAC21050.24534142668662032No Hit
GCCCCAGAGCTGGAGCCTGAGAAACGATCAGGAACTCCGCTCCCCTTGTT20530.2392807358611076No Hit
GTACTTGATGAAGCTTGAAGGTAGTGGAAGTTACAACAAGGGGAGCGGAGTTCCTGATCGTTTCTCAGGCTCCAG19800.23077245835606092No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19690.2294903891429717No Hit
CACCTGTACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATC19220.22401245705068137No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18340.21375590334596753No Hit
GTATATTACTGTGCGAAATCGGCGAGTGATAGTCGCTACTGGGGCCAGGG17830.20781176426709932No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17780.2072290055338769No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16920.19720555532245207No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16660.19417520990969572No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16060.1871821051110272No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15780.18391865620498188No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15700.18298624223182608No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15640.18228693175195923No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15020.1750607234600018No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14830.17284624027375675No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14820.17272968852711226No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14760.1720303780472454No Hit
GCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAG14560.16969934311435592No Hit
CCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGT14440.16830072215462222No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14320.16690210119488852No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14060.16387175578213214No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14010.16328899704890978No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13980.16293934180897635No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC13600.1585103754364863No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13580.15827727194319735No Hit
GTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGG13460.15687865098346362No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13140.15314899509084043No Hit
GAATAACAGGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAGCCTCAT12970.15116761539788437No Hit
GCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGAGAC12920.15058485666466198No Hit
CAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTC12370.14417451059921582No Hit
TCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGCCTGAGAAACGATCAGGAACT12170.14184347566632635No Hit
ATCAAGTACCGAGGGGCCTTCCCTGGCTGCTGCTGATGCCATGCGATGAT12160.14172692391968186No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12160.14172692391968186No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12070.14067795819988158No Hit
GAGCTGGAGCCTGAGAAACGATCAGGAACTCCGCTCCCCTTGTTGTAACT12020.14009519946665921No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11800.13753106104048074No Hit
GTATTAGTGTTAGTGGTAGGAGCACATACTACGCAGACTCCCTGAAGGGCCGGTTCACCATCTCCAGAGACAATT11650.13578278484081363No Hit
GTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGTACTCTGAGCAGTGGG11490.13391795689450203No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11460.1335683016545686No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAGGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAGC11390.13275243942805726No Hit
CAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGTACTCTGAGCAGTGGGCACAGTAG11340.1321696806948349No Hit
GGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAGCCTCATCCTCAGAC11300.131703473708257No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11030.12855657654885616No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAGGGTGTTC10980.1279738178156338No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATC10830.12622554161596666No Hit
GCTTGGTCCCTCCGCCGAATAACAGGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAGCCTCATCCTCAGA10460.12191312699012108No Hit
GACTGGAGGTTGGAGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGCCTGAGAAACGATCAGGAACTCCGCTC10260.11958209205723157No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10250.11946554031058709No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10250.11946554031058709No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10240.11934898856394262No Hit
GTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCAC10180.11864967808407577No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10170.1185331263374313No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGC10160.1184165745907868No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG10100.11771726411091996No Hit
GAGCACATACTACGCAGACTCCCTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTAT10080.11748416061763102No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10020.11678485013776416No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9920.11561933267131941No Hit
GGGAAGGCCCCTCGGTACTTGATGAAGCTTGAAGGTAGTGGAAGTTACAA9880.11515312568474152No Hit
CTACCTCACCATCTCCAACCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGGAACACC9850.11480347044480808No Hit
GTATGTGCTCCTACCACTAACACTAATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT9840.11468691869816361No Hit
CACTAATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC9840.11468691869816361No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9700.11305519424514095No Hit
GGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGA9700.11305519424514095No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9690.11293864249849647No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGGAACACCCTGTTATTCGGCGGAGGGACCAAG9560.11142346979211831No Hit
GGAACACCCTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT9550.11130691804547384No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9430.10990829708574014No Hit
ACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAGCAGCA9400.1095586418458067No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9310.10850967612600643No Hit
CCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGGAACACCCTGTTATTCGGCGGAGGGAC9290.10827657263271746No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9210.10734415865956168No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9150.10664484817969483No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9100.10606208944647244No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9070.10571243420653902No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9020.10512967547331663No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9020.10512967547331663No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8970.10454691674009428No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8900.10373105451358293No Hit
GTACAGGTGAGCTTGACCGAGGATCCCAGGGAAGCAGAGGCAGAGGATGA8900.10373105451358293No Hit
GTATCAACGCAGAGTACGGGAGCAGAGCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTC8880.103497951020294No Hit
AGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGTA8680.10116691608740447No Hit
CTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG8660.10093381259411553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1750.054.7100751
TCTGGAG8900.052.49197470
TGGTATC1700.052.29642
AGGGGGG1900.052.157170
CGTATGC1400.041.90942841
GGTATCA5400.041.792421
TATACAC1400.041.5210383
TACTAGA1350.040.6017131
ATGCCGT1450.040.5956344
GACCGTA1350.040.54725626
TCGTATG1400.039.39781640
ACCGTAC1400.039.10597627
TCTCGTA1150.038.90328238
GTATCAA12550.038.4169351
GACAGCC1700.038.21661
GCCGTCT1550.038.08670846
TTATACA1550.037.5028762
CTTATAC1550.037.5028761
TATAAGA557.85842E-637.3023222
ACTAGAT1400.036.73255532
Produced by FastQC (version 0.11.5)