FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004688658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857988
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATCAG33460.38998214427241407No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28060.3270442010843974No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAG27950.32576213187130826No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGC27680.3226152347119074No Hit
CTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGTACT26470.3085124733679259No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26450.30827936987463694No Hit
GATTATTACTGTGAGACCTGGGACAGGAACACCCTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG25640.29883867839643447No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24990.2912628148645436No Hit
GCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGT24600.286717296745409No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA23650.27564488081418387No Hit
GGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCAC23240.2708662592017604No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAGGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAG23120.2694676382420267No Hit
GAGTACAGGTGAGCTTGACCGAGGATCCCAGGGAAGCAGAGGCAGAGGAT19840.23123866534263882No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19550.22785866468994906No Hit
GTACTTGATGAAGCTTGAAGGTAGTGGAAGTTACAACAAGGGGAGCGGAGTTCCTGATCGTTTCTCAGGCTCCAG18600.21678624875872388No Hit
CACCTGTACTCTGAGCAGTGGGCACAGTAGCTACATCATCGCATGGCATC18090.21084210967985567No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18050.21037590269327777No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17340.20210072868152001No Hit
GCCCCAGAGCTGGAGCCTGAGAAACGATCAGGAACTCCGCTCCCCTTGTT17240.20093521121507524No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16870.19662279658922968No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16100.1876483120976051No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15960.18601658764458243No Hit
GTATATTACTGTGCGAAATCGGCGAGTGATAGTCGCTACTGGGGCCAGGG15790.18403520795162637No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15130.176342792673091No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14880.1734289990069791No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14310.16678554944824403No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14260.16620279071502167No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14260.16620279071502167No Hit
GCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAG14150.16492072150193243No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14140.16480416975528794No Hit
GGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAGCCTCATCCTCAGAC13690.1595593411562866No Hit
CCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGT13670.1593262376629976No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13480.1571117544767526No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13260.15454761605057413No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13250.15443106430392967No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12970.15116761539788437No Hit
GTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGG12590.1467386490253943No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12450.14510692457237165No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12380.1442910623458603No Hit
GCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGAGAC12270.1430089931327711No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11920.13892968200021444No Hit
GTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGTACTCTGAGCAGTGGG11910.13881313025356998No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11660.1358993365874581No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11350.13228623244147936No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11340.1321696806948349No Hit
GTATTAGTGTTAGTGGTAGGAGCACATACTACGCAGACTCCCTGAAGGGCCGGTTCACCATCTCCAGAGACAATT11110.12948899052201196No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10950.12762416257570036No Hit
GAATAACAGGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAGCCTCAT10920.12727450733576695No Hit
CAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGTACTCTGAGCAGTGGGCACAGTAG10850.1264586451092556No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10810.1259924381226777No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10800.12587588637603322No Hit
GAGCTGGAGCCTGAGAAACGATCAGGAACTCCGCTCCCCTTGTTGTAACT10720.12494347240287743No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10710.12482692065623295No Hit
ATCAAGTACCGAGGGGCCTTCCCTGGCTGCTGCTGATGCCATGCGATGAT10580.12331174794985478No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10570.1231951962032103No Hit
CAGTAGCTACATCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTC10550.12296209270992134No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10530.1227289892166324No Hit
TCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGCCTGAGAAACGATCAGGAACT10510.12249588572334345No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATC10280.11981519555052053No Hit
GAGCACATACTACGCAGACTCCCTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTAT10130.1180669193508534No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10090.11760071236427548No Hit
GTCTCTCTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCAC10090.11760071236427548No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGGAACACCCTGTTATTCGGCGGAGGGACCAAG9950.11596898791125285No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG9890.115269677431386No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAGGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAGC9810.11433726345823017No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9790.11410415996494123No Hit
GTATCAACGCAGAGTACGGGAGCAGAGCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTC9780.11398760821829676No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9640.1123558837652741No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAGGGTGTTC9590.11177312503205172No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9590.11177312503205172No Hit
GGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGA9460.11025795232567355No Hit
GACTGGAGGTTGGAGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGCCTGAGAAACGATCAGGAACTCCGCTC9400.1095586418458067No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9360.10909243485922879No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9250.10781036564613958No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGCGGTCAGCCCCAGAGCTGGAGC9150.10664484817969483No Hit
GGAACACCCTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT9110.10617864119311692No Hit
GTATGTGCTCCTACCACTAACACTAATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT8930.10408070975351637No Hit
GCTTGGTCCCTCCGCCGAATAACAGGGTGTTCCTGTCCCAGGTCTCACAGTAATAATCAGCCTCATCCTCAGA8860.10326484752700504No Hit
CTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCCTGTGCTGACTCAATC8850.10314829578036055No Hit
GGGAAGGCCCCTCGGTACTTGATGAAGCTTGAAGGTAGTGGAAGTTACAA8850.10314829578036055No Hit
CCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGAGACCTGGGACAGGAACACCCTGTTATTCGGCGGAGGGAC8740.10186622656727135No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8610.10035105386089316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)