FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786116
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT35030.4456085361447929No Hit
CTATTAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTC29550.37589872232596716No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28740.3655948994804838No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24540.3121676699113108No Hit
GTGATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA21220.2699347170137741No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21190.2695530939454228No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19290.24538363294984455No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT17650.224521571879977No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17330.22045092581756384No Hit
CTGTATCACTGTGCGAGAGATCCAGCAGGCCGCCCCATTTATGACTCCTGGGGCCAGGGAACCCTGGTCACCGT17160.21828839509690684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16990.21612586437624984No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15130.19246523413847322No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15010.19093874186506826No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14990.19068432648616743No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA14640.18623205735540302No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14510.1845783573925477No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAG14370.1827974497402419No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14000.17809076523057665No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13940.1773275190938742No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13700.1742745345470643No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT13590.17287524996310977No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12890.16397071170158095No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12760.1623170117387256No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12530.1593912348813661No Hit
GTATCACTGTGCGAGAGATCCAGCAGGCCGCCCCATTTATGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC12470.15862798874466366No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12330.15684708109235787No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12070.15353968116664715No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11860.1508683196881885No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11830.15048669661983727No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11720.14908741203588274No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11540.14679767362577534No Hit
CAGTGATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATA11510.1464160505574241No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG11290.14361748138951505No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG11220.14272702756336214No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11170.1420909891161101No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10610.13496735850688704No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC10340.13153275089172592No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10180.12949742786051932No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT10160.12924301248161849No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG10140.12898859710271765No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9580.12186496649349458No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9510.12097451266734172No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9480.12059288959899049No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9460.12033847422008966No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9280.11804873580998224No Hit
ACTTATTACTGTCAACAGTTTAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA9280.11804873580998224No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCTCT9240.11753990505218058No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9230.11741269736273018No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA9200.11703107429437895No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9120.11601341277877565No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8940.11372367436866823No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8900.11321484361086658No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG8850.11257880516361454No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATCCAATAGGAAC8850.11257880516361454No Hit
GATTTCTCTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTAATAGTTAC8820.11219718209526329No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8790.11181555902691205No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8710.11079789751130877No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8690.11054348213240794No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGTGATAAATTA8660.1101618590640567No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGATCCACTGCCG8640.10990744368515588No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8590.10927140523790382No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG8280.10532796686494104No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC8180.10405588997043694No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8160.1038014745915361No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8120.10329264383373447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)