FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799592
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54880.30495801270510203No Hit
GCCTTGGGCTGACCTAGGGCGGTCAGTCTGGTCCCTCCGCCGAACACCACTTGACCACTATCACTGTCCCACAG50600.28117484407576826No Hit
CCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46370.2576695162014501No Hit
GACCTAGGGCGGTCAGTCTGGTCCCTCCGCCGAACACCACTTGACCACTA43880.24383304660167415No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41970.23321952976007895No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41090.2283295324718047No Hit
GTATTGGTCGTCTATAATGATAAGAATCGGCCCTCAGGGATCTCTGAGCG38680.2149376080800537No Hit
GTAATAGACAGCCGTGTCCGCGGCAGTCACAGAGGTCAGCCTCAGGGAGA37580.20882511146971092No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35260.19593330043698792No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGGCGGTCAGTCTGGTCCCTC35200.1955998915309692No Hit
CTATTACTGTCAGCTGTGGGACAGTGATAGTGGTCAAGTGGTGTTCGGCG33630.18687569182348No Hit
CCCCAGGACAGACGGCCACAATTACTTGTGGGGGAGACAACATTGGACCT32520.18070762706213409No Hit
GGCCAGGCCAGGCCCCTGTATTGGTCGTCTATAATGATAAGAATCGGCCC31940.17748467430395334No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31090.17276138146868847No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30890.17165001844862612No Hit
GTCTGGTCCCTCCGCCGAACACCACTTGACCACTATCACTGTCCCACAGCTGACAGTAATAGTCGGCCGCATCCC30840.17137217769361057No Hit
CCCCTGTATTGGTCGTCTATAATGATAAGAATCGGCCCTCAGGGATCTCT30630.17020524652254512No Hit
CAGTAATAGTCGGCCGCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGT30430.16909388350248278No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29960.16648218040533633No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT29850.16587093074430204No Hit
CGTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG29480.16381490915718674No Hit
CTCTGTGACCTCGTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCC29370.16320365949615245No Hit
GGACCAGACTGACCGCCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG28570.15875820741590316No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCGTATGTGCTGACT27870.15486843684568502No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27560.1531458241645884No Hit
CTATCACTGTCCCACAGCTGACAGTAATAGTCGGCCGCATCCCCGGCCTC26980.14992287140640767No Hit
CTTTTAGGTCCAATGTTGTCTCCCCCACAAGTAATTGTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG26730.14853366763132977No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26280.14603310083618953No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25650.1425323073229932No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25650.1425323073229932No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25570.14208776211496826No Hit
GGCCAGGCCCCTGTATTGGTCGTCTATAATGATAAGAATCGGCCCTCAGG25490.14164321690694337No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25480.14158764875594024No Hit
ATACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACACTTTTAGGT25110.13953162716882495No Hit
CTGCACAGACTCTGTGACCTCGTATGTGCTGACTCAGCCACCCTCGGTGT24450.13586412920261925No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGA23740.131918790481398No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23510.13064072300832633No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23320.12958492813926714No Hit
ACCTAAAAGTGTGCACTGGTACCAGCAGAGGCCAGGCCAGGCCCCTGTAT23230.1290848147802391No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23040.12802901991117988No Hit
GTCTATTACTGTGTGAGACCTCTTCAGGCCCTATCCGGACTCTACTTTGA22950.12752890655215182No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCGTATGTGCTGACTCAGC22690.1260841346260708No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22590.12552845311603963No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22300.12391697673694926No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21890.12163868254582148No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21230.11797118457961583No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG20120.11180311981826992No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG19880.11046948419419512No Hit
ACATTGGACCTAAAAGTGTGCACTGGTACCAGCAGAGGCCAGGCCAGGCC19860.11035834789218889No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19850.11030277974118578No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19760.10980266638215773No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19680.1093581211741328No Hit
CAATTACTTGTGGGGGAGACAACATTGGACCTAAAAGTGTGCACTGGTACCAGCAGAGGCCAGGCCAGGCCC19570.10874687151309853No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19450.10808005370106114No Hit
GCACAGACTCTGTGACCTCGTATGTGCTGACTCAGCCACCCTCGGTGTCA19400.10780221294604554No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC19380.1076910766440393No Hit
GTGATAGTGGTCAAGTGGTGTTCGGCGGAGGGACCAGACTGACCGCCCTA19330.10741323588902374No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT19180.106579713623977No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCGTA18920.10513494169789596No Hit
GTCTCTGGTGACTCCATCCGCAGTGGCACTTACTACGGGGGCTGGATTCG18920.10513494169789596No Hit
CAGTGATAGTGGTCAAGTGGTGTTCGGCGGAGGGACCAGACTGACCGCCC18770.10430141943284922No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCGTATGTGCTGACTCAGCCAC18700.10391244237582742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6600.050.7036770
GGTATCA18550.044.5904241
CGTATGC6300.039.44962341
GACGTAG7250.038.68745826
CGAGACG7550.037.99887523
CGTAGAG7400.037.9507728
ATGCCGT6350.037.73665644
ACGTAGA7550.037.6218827
AATCTCG6100.036.94769736
GTGGTAT4250.036.5416641
ATCTCCG7850.036.54160710
GTATCAA47450.036.3583451
CCTAACG656.472055E-736.350262
CTAACGC656.472055E-736.350263
AGACGTA7750.036.16935325
CACGAGA7750.036.1432221
TCTCGTA5700.036.0788238
TATACAC8150.036.0316773
ACGAGAC7900.035.88714222
ATCTCGT6200.035.86653537
Produced by FastQC (version 0.11.5)