FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799592
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74890.41614988286233767No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52150.2897879074812513No Hit
GCCTTGGGCTGACCTAGGGCGGTCAGTCTGGTCCCTCCGCCGAACACCACTTGACCACTATCACTGTCCCACAG48050.26700496556997366No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43650.2425549791286025No Hit
CCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40280.22382851224055234No Hit
GACCTAGGGCGGTCAGTCTGGTCCCTCCGCCGAACACCACTTGACCACTA39340.21860510604625938No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38840.2158266984961036No Hit
GTATTGGTCGTCTATAATGATAAGAATCGGCCCTCAGGGATCTCTGAGCG38440.2136039724559789No Hit
GTAATAGACAGCCGTGTCCGCGGCAGTCACAGAGGTCAGCCTCAGGGAGA36540.20304602376538683No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35880.19937852579918117No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33300.18504194284037714No Hit
GTCTGGTCCCTCCGCCGAACACCACTTGACCACTATCACTGTCCCACAGCTGACAGTAATAGTCGGCCGCATCCC32170.17876274177702503No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGGCGGTCAGTCTGGTCCCTC31900.17726240169994087No Hit
CTATTACTGTCAGCTGTGGGACAGTGATAGTGGTCAAGTGGTGTTCGGCG31620.1757064934718536No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30880.17159445029762302No Hit
GGCCAGGCCAGGCCCCTGTATTGGTCGTCTATAATGATAAGAATCGGCCC30730.17076092803257628No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30700.1705942235795669No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30150.16753797527439554No Hit
CCCCTGTATTGGTCGTCTATAATGATAAGAATCGGCCCTCAGGGATCTCT30040.16692672561336125No Hit
CCCCAGGACAGACGGCCACAATTACTTGTGGGGGAGACAACATTGGACCT29750.16531524923427088No Hit
CTCTGTGACCTCGTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCC28720.1595917296809499No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCGTATGTGCTGACT28480.15825809405687513No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27850.15475730054367878No Hit
CGTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG26890.14942275804737962No Hit
CTTTTAGGTCCAATGTTGTCTCCCCCACAAGTAATTGTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG26590.14775571351728614No Hit
CAGTAATAGTCGGCCGCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGT25990.14442162445709916No Hit
ATACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACACTTTTAGGT25780.1432546932860337No Hit
GGCCAGGCCCCTGTATTGGTCGTCTATAATGATAAGAATCGGCCCTCAGG25080.13936492271581558No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGA24860.13814242339374702No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCGTATGTGCTGACTCAGC24780.1376978781857221No Hit
CTGCACAGACTCTGTGACCTCGTATGTGCTGACTCAGCCACCCTCGGTGT24730.1374200374307065No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24710.13730890112870028No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24540.1363642425616473No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24340.135252879541585No Hit
CTATCACTGTCCCACAGCTGACAGTAATAGTCGGCCGCATCCCCGGCCTC23660.13147424527337306No Hit
GGACCAGACTGACCGCCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG23370.1298627688942827No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23090.12830686066619545No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23080.12825129251519235No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22940.1274733384011487No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22570.1254173168140334No Hit
GTCTATTACTGTGTGAGACCTCTTCAGGCCCTATCCGGACTCTACTTTGA22180.12325015892491187No Hit
ACCTAAAAGTGTGCACTGGTACCAGCAGAGGCCAGGCCAGGCCCCTGTAT21400.1189158431466688No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21090.1171932304655722No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20920.11624857189851921No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCGTA20680.11491493627444442No Hit
GTGATAGTGGTCAAGTGGTGTTCGGCGGAGGGACCAGACTGACCGCCCTA20460.11369243695237587No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20380.11324789174435093No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC20270.11263664208331667No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG19860.11035834789218889No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT19470.10819119000306736No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19440.108024485550058No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19380.1076910766440393No Hit
ACATTGGACCTAAAAGTGTGCACTGGTACCAGCAGAGGCCAGGCCAGGCC19350.10752437219102996No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19210.10674641807698634No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18840.10469039648987105No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18510.1028566475067682No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18280.10157858003369653No Hit
GCACAGACTCTGTGACCTCGTATGTGCTGACTCAGCCACCCTCGGTGTCA18230.10130073927868094No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTATTGGTCGTCTATAATGAT18090.10052278516463732No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18020.10013380810761549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)