FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088727
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64270.5903224591656128No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58820.5402639963921166No Hit
GTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTT52380.4811123449680223No Hit
CTACAAACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG44910.41250010333168924No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42410.38953750572916807No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG41450.3807198682498No Hit
GTAGTACACAGCCGTGTCGTCGCCTCGCAGGCTGTTCATTTGCAGATATA39920.36666675851705705No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37860.34774557809257967No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37250.34214270427756455No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36820.3381931374899309No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35980.3304777046954838No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34590.3177105004284821No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32490.2984219184423643No Hit
GGGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT31980.29373754853145No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC31910.29309459579857944No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30970.2844606591000315No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30850.2833584544151105No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA30450.2796844387987071No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT30390.2791333364562466No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29360.26967274624400794No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28280.2597529040797188No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG27130.2491901091825591No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26820.24634274707984646No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA26630.24459758966205486No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26190.24055617248401115No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC25960.2384436135045792No Hit
AGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCC25230.23173853500464306No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25190.2313711334430027No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAAACC25050.23008522797726152No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23450.215389165511648No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22880.21015369325827318No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22870.2100618428678631No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT22250.20436711866243787No Hit
GTTTATTACTGCATGCAAACTCTACAAACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA21630.19867239445701262No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21620.19858054406660255No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21320.19582503235430002No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC20650.18967105619682437No Hit
GTCAGGGACCCCGGAGGCCCGACTAGAACCCAAAAAGATCAGGAGATGTGGAGACTGCCCTGGCTTCTGCAGGTA20590.18911995385436386No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA20520.18847700112149327No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20360.18700739487493193No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20160.18517038706673022No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC20010.18379263121057898No Hit
GTCTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCCTCCGGGGTCC19720.18112896988868651No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19500.17910826129966465No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19450.17864900934761424No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG19360.17782235583392347No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19100.1754342456832613No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18910.17368908826546967No Hit
CTCTACAAACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA18670.17148467889562766No Hit
GGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTTCTAGT18640.1712091277243974No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18510.1700150726490663No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC18450.1694639703066058No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17780.1633099941491301No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17770.16321814375872004No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG17720.1627588918066696No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17700.16257519102584947No Hit
TTATTACTGCATGCAAACTCTACAAACCCCGCTCACTTTCGGCGGAGGGA17580.16147298634092844No Hit
GCATAGTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGC17540.1611055847792881No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA17360.15945227775190657No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT17290.158809325019036No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17230.1582582226765755No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17140.15743156916288473No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16980.1559619629163234No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAT16680.15320645120402085No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16620.15265534886156035No Hit
CTCCTGCATAGTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGAT16510.1516449945670494No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16490.15146129378622925No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16230.14907318363556704No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAAACCCCGCTCACTTTCGGCGGAGGGA15990.146868774265725No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15900.14604212075203427No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15810.1452154672383435No Hit
CTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCCTCCGGGGTCCCT15610.1433784594301418No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT15520.14255180591645106No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15470.14209255396440063No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15380.14126590045070986No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15180.13942889264250818No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15060.13832668795758718No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA14930.13713263288225608No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14840.1363059793685653No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14840.1363059793685653No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14790.13584672741651488No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAAAC14650.1345608219507737No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14380.13208086140970143No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14300.13134605828642074No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14100.12950905047821903No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT14000.12859054657411823No Hit
GGCATACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGG13990.12849869618370813No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13980.12840684579329803No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13970.12831499540288796No Hit
GCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTT13820.1269372395467367No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATGGTTGTATCCATTACTATGC13810.1268453891563266No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTA13800.12675353876591652No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13630.1251920821289451No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGA13560.12454912939607449No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13530.12427357822484425No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC13420.12326322393033332No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13410.12317137353992323No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13290.12206916885500223No Hit
GTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT13060.11995660987557029No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC12910.118578854019419No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12680.11646629503998707No Hit
CAGTAGTACACAGCCGTGTCGTCGCCTCGCAGGCTGTTCATTTGCAGATA12400.11389448410850471No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12340.11334338176604421No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGC12330.11325153137563412No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12230.11233302747153326No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT12120.11132267317702234No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA12070.11086342122497192No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11810.10847531107430972No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG11800.10838346068389965No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11620.10673015365651813No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11200.10287243725929456No Hit
ATGTTGGGGTTTATTACTGCATGCAAACTCTACAAACCCCGCTCACTTTC11140.10232133491683405No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11130.10222948452642398No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11120.1021376341360139No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTC10990.1009435790606828No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA10950.10057617749904246No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10940.10048432710863238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)