FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088727
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64090.5886691521382312No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47800.4390448661602036No Hit
GTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTT45160.41479636309194134No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42760.39275226939352104No Hit
CTACAAACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG39780.3653808530513159No Hit
GGGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT39370.3616149870445024No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37930.34838853082545024No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37540.344806365599457No Hit
GTAGTACACAGCCGTGTCGTCGCCTCGCAGGCTGTTCATTTGCAGATATA36920.3391116413940317No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35160.3229459726818569No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG34420.31614904379151065No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33960.3119239258326468No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA32320.2968604618053929No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC31350.2879509739356147No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30330.2785822341137861No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29080.26710093531252554No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28770.2642535732098129No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT27940.2566299908057759No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27770.2550685341688045No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27050.24845530605927837No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26970.24772050293599773No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26470.2431279834154935No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26150.24018877092237081No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25460.23385109398407497No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25380.2331162908607943No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24750.22732971626495901No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAAACC24600.22595196040880772No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24160.221910543230764No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA24100.22135944088830348No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC23500.21584841746369843No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23450.215389165511648No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23380.2147462127787774No Hit
AGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCC21460.1971109378200412No Hit
GTTTATTACTGCATGCAAACTCTACAAACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA20970.19261026868994707No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT20950.1924265679091269No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20780.19086511127215547No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20460.18792589877903276No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC19160.1759853480257218No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19010.1746075921695705No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18930.17387278904628983No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18890.1735053874846495No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG18850.17313798592300916No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18800.17267873397095876No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC18760.1723113324093184No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18380.16882101757373522No Hit
GTCAGGGACCCCGGAGGCCCGACTAGAACCCAAAAAGATCAGGAGATGTGGAGACTGCCCTGGCTTCTGCAGGTA18320.1682699152312747No Hit
CTCTACAAACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA18280.16790251366963435No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA18260.16771881288881418No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18080.16606550586143268No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG17860.1640447972724108No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17690.16248334063543937No Hit
GGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTTCTAGT17650.16211593907379904No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA17590.16156483673133853No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17380.15963597853272676No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17310.15899302579985616No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA17290.158809325019036No Hit
CTCCTGCATAGTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGAT17260.15853377384780573No Hit
TTATTACTGCATGCAAACTCTACAAACCCCGCTCACTTTCGGCGGAGGGA17240.15835007306698556No Hit
GTCTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCCTCCGGGGTCC17010.15623751408755363No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16950.15568641174509312No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16360.15026723871089814No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC16280.14953243558761745No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT16200.1487976324643368No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAAACCCCGCTCACTTTCGGCGGAGGGA15580.14310290825891156No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15550.1428273570876813No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15510.14245995552604096No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15410.14154145162194012No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15330.14080664849865945No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15320.14071479810824936No Hit
GCATAGTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGC15230.13988814459455862No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15200.13961259342332835No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15190.13952074303291825No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTACAAAC14930.13713263288225608No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14920.13704078249184598No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAT14860.13648968014938548No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14810.13603042819733507No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14550.13364231804667287No Hit
GTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT14290.13125420789601067No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13890.1275801922796073No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATGGTTGTATCCATTACTATGC13850.12721279071796696No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13840.12712094032755686No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13840.12712094032755686No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13820.1269372395467367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13730.12611058603304595No Hit
CTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCCTCCGGGGTCCCT13690.1257431844714056No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13660.12546763330017535No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT13620.125100231738535No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13590.12482468056730475No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13580.12473283017689467No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13520.12418172783443415No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13370.12280397197828291No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13290.12206916885500223No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13220.12142621612213163No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13150.12078326338926103No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13090.12023216104680053No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC13010.11949735792351984No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12980.1192218067522896No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12770.11729294855367782No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAACTCTA12680.11646629503998707No Hit
GGCATACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGG12590.1156396415262963No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12420.11407818488932486No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC12400.11389448410850471No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12150.1115982243482526No Hit
GCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTT12080.110955271615382No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA12060.11077157083456182No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGC12030.11049601966333158No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT11870.10902641341677022No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11840.10875086224553998No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT11290.10369909077298534No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTTTTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGA11290.10369909077298534No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11220.10305613804011474No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG11000.10103542945109288No Hit
CAGTAGTACACAGCCGTGTCGTCGCCTCGCAGGCTGTTCATTTGCAGATA11000.10103542945109288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT350.005359808456.06435870
GGTATCA10150.047.5536881
GTGGTAT2250.045.6425441
TCTGGAG13550.044.41014570
GTATCAA27150.038.7079051
GTTATAC451.03561506E-438.11606628
TGGTATC2700.038.0109862
ATCAACG32000.033.0217863
TATCAAC32150.032.87982
TCAACGC32300.032.8209574
CAACGCA33100.032.026235
AACGCAG33150.031.9779226
CTCGCTA652.4385608E-531.6175823
AACCCGG850.00453780530.7804370
ACGCAGA34650.030.4949077
CGCAGAG34600.030.4401448
ATACGTA1700.030.17271811
CGTACCA1600.029.9212814
AGAGTAC35650.029.4476711
GTGCTAG1750.029.3416351
Produced by FastQC (version 0.11.5)