FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583086
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16350.2804046058385899No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC12230.2097460751930247No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT12110.20768805973732865No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG9480.1625832209999897No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA9140.15675217720885085No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8470.14526159091454777No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC7510.1287974672689792No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC7210.123652428629739No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGG6900.11833588870252415No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC6860.11764988355062547No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC6520.11181883975948659No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG6520.11181883975948659No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA6500.11147583718353726No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT6420.11010382687973988No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6320.10838881399999314No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6060.10392978051265164No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC5850.10032825346518352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3300.059.78339470
GATAACG200.006695965352.99687646
CGATAAC200.00677364852.8425545
CGCGTCC200.008553749.81442617
GTGATAC200.00856240449.801613
CGTATGC3050.047.87082341
TCGTATG3050.047.6824240
ATGCCGT3050.047.23151444
CTCGTAT2650.046.83453439
TGCCGTC3150.045.85279545
CGAGACA3300.043.29170623
GCCGTCT3450.043.01195546
CACGAGA3500.041.7635621
CCGTCTT3600.041.40875247
ACGAGAC3450.041.40590322
ATCTCGT3000.041.03430637
GTATGCC3600.040.7144442
CCCACGA3450.040.4256319
GGCAATC3050.039.8755433
ATCGTAT350.001351958139.40048239
Produced by FastQC (version 0.11.5)