FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161144
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAGCAATAAGAACTACTTAGCTTGGTACCAGCTGAA63490.5467883397752562No Hit
CTCCTAACCTGCTCATCTACTGGGCATCTATTCGGGAATCCGGGGTCCCT56080.4829719655787741No Hit
GGTTAGGAGGCTGCCCTGGTTTCAGCTGGTACCAAGCTAAGTAGTTCTTATTGCTGGAGCTGTATAAAACACT53790.46325003617122423No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50400.4340546908910523No Hit
ATATTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA50210.432418373603963No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA47200.4064956628979696No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45980.39598878347560684No Hit
GAGTAATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT44550.38367334284119803No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40400.34793272841266887No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34440.2966040387755524No Hit
ATATTATATTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT34070.2934175261638522No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33490.288422452340106No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31720.2731788649814321No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30490.2625858635965909No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29160.25113164258696596No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28790.24794512997526577No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27610.23778273840281655No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27610.23778273840281655No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27550.23726600662794622No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27130.23364888420385413No Hit
ATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC26640.22942890804241337No Hit
GTTTATTACTGTCAGCAATATTATATTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA26580.22891217626754304No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25850.22262527300662105No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25270.21763019918287482No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25060.21582163797082876No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21940.18895158567757314No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21740.18722914642800548No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21530.18542058521595942No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21240.18292304830408632No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21220.18275080437912955No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAG21180.18240631652921602No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA21170.18232019456673762No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21130.1819757067168241No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20790.17904755999255906No Hit
GTAGATGAGCAGGTTAGGAGGCTGCCCTGGTTTCAGCTGGTACCAAGCTA20780.1789614380300807No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20700.17827246233025362No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20680.17810021840529686No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20140.17344963243146413No Hit
GTCAGGGACCCCGGATTCCCGAATAGATGCCCAGTAGATGAGCAGGTTAGGAGGCTGCCCTGGTTTCAGCTGGTA19910.17146882729446133No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19800.1705214857071991No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACT19110.16457907029619065No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18930.16302887497157975No Hit
GGTTTCAGCTGGTACCAAGCTAAGTAGTTCTTATTGCTGGAGCTGTATAA18590.1601007282473147No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18280.15743094741048483No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC18010.1551056544235685No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17640.1519191418118683No Hit
GCTGAAACCAGGGCAGCCTCCTAACCTGCTCATCTACTGGGCATCTATTCGGGAATCCGGGGTCCCTGACCGATT17620.15174689788691154No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17450.150282824524779No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17390.1497660927499087No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC17350.14942160489999518No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17080.14709631191307881No Hit
GCTCATCTACTGGGCATCTATTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT17060.14692406798812208No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16420.14141226238950552No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16050.13822574977780533No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAGCAATAAGAACTA15790.13598657875336737No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15770.1358143348284106No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15620.13452250539123486No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15600.1343502614662781No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15590.1342641395037997No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15440.13297231006662397No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15390.13254170025423204No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15380.13245557829175364No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14940.12866621194270478No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14710.12668540680570195No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14620.12591030914339651No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14500.1248768455936559No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14500.1248768455936559No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14480.12470460166869915No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14430.12427399185630722No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14280.12298216241913149No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14240.12263767456921794No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14240.12263767456921794No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14190.12220706475682604No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14050.12100135728212866No Hit
GCTTGGTACCAGCTGAAACCAGGGCAGCCTCCTAACCTGCTCATCTACTGGGCATCTATTCGGGAATCCGGGGTC14000.12057074746973674No Hit
CTACTTAGCTTGGTACCAGCTGAAACCAGGGCAGCCTCCTAACCTGCTCATCTACTGGGCATCTATTCGGGAATC13990.12048462550725836No Hit
GAACTACTTAGCTTGGTACCAGCTGAAACCAGGGCAGCCTCCTAACCTGC13960.1202262596198232No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACTCCGT13840.11919279607008261No Hit
GAATCGGTCAGGGACCCCGGATTCCCGAATAGATGCCCAGTAGATGAGCAGGTTAGGAGGCTGCCCTGGTTTCAG13690.11790096663290685No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13560.11678138112068788No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13560.11678138112068788No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACTCCGTGGACGTT13420.1155756736459905No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13410.11548955168351213No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13380.11523118579607697No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13350.11497281990864182No Hit
ACTTAGCTTGGTACCAGCTGAAACCAGGGCAGCCTCCTAACCTGCTCATC13260.11419772224633637No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13130.1130781367341174No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12690.10928877038506853No Hit
CCCGAATAGATGCCCAGTAGATGAGCAGGTTAGGAGGCTGCCCTGGTTTC12680.10920264842259014No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12530.10791081898541438No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12530.10791081898541438No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12460.1073079652480657No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC12450.10722184328558731No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12410.10687735543567378No Hit
GTAATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT12400.1067912334731954No Hit
GCTAAGTAGTTCTTATTGCTGGAGCTGTATAAAACACTCTGGCTGGACTT12330.10618837973584673No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12200.10506879422362773No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG12180.10489655029867095No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12150.10463818441123582No Hit
GTACCAAGCTAAGTAGTTCTTATTGCTGGAGCTGTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCT11960.10300186712414654No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC11930.10274350123671137No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAGTAATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC11730.10102106198714371No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAAT11630.10015984236235989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCGA250.00111707583.2662570
ATATCGT150.002422560768.5135737
AATATCG208.865298E-568.4391736
GTGGTAT2300.047.211
GGTATCA9200.046.103511
CGTATGC2350.045.48647741
TCGTATG2300.044.9171340
CTTATAC2900.042.1227151
TTCACGG3500.041.63312570
ATGCCGT2500.041.5526144
AATCTCG2150.041.38182436
TGGTATC2650.040.9747162
TATGCCG2750.040.23155243
TCTCCGA3050.040.0442111
GTATGCC2700.039.64386742
ATCTCCG3100.039.3983310
TCCGAGC3200.039.22733313
CGAGACA3300.039.11048523
CCGTCTT2500.038.95192347
CACGAGA3250.038.648821
Produced by FastQC (version 0.11.5)