FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108520
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGGGGGGGACGTTCGGCCAAGGGACCAAGGTGG45760.41280265579331No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41320.3727492512539241No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41310.37265904088333995No Hit
GTTATTATACTGCTGACAGTAATAAACTGCTAAATCTTCAGACTGCAGGCTGCTGATGGTGAGACTGAACT32790.29579980514559956No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30690.276855627322917No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29110.2626023887706131No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGGGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA27320.24645473243604085No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25570.23066791758380545No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25030.22579655757225847No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24780.22354129830765346No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24730.22309024645473247No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24570.2216468805253852No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23570.21262584346696495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21740.19611734565005592No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21490.19386208638545088No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCAGCCTGCAGTCTGAAGATT21430.19332082416194565No Hit
CTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGT20770.1873669397033883No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20060.18096200339190993No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19910.1796088478331469No Hit
GTAATAAACTGCTAAATCTTCAGACTGCAGGCTGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA19790.17852632338613647No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18840.16995633818063724No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTTTGCAGGGCCAGTCAGAGTGTTAACCG18710.16878360336304263No Hit
GTGTATTACTGTGCGAGAGATCTGGCGGTGACTACAGTCTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGT18690.1686031826218742No Hit
GTAATACACAGCCGTATCTTCAAATCTCAGGCTCCTCAGCTCCATGTAGG17930.16174719445747485No Hit
GAGTTCAGTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTAGCAGTTTATTACTGTCAGCAGTATAATAACTGG17920.16165698408689064No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17620.15895067296936458No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17550.15831920037527514No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16990.15326741962255983No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16990.15326741962255983No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTTTGCAGGGCCAGTCAGAGTGTTAACCGCAACTTAGCCTGGTA16910.15254573665788618No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGTGGCCCTGGT16600.1497492151697759No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCCCCCCGGAGG16590.14965900479919172No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG15560.14036733662901887No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15500.13982607440551367No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15380.13874354995850322No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATGGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG14760.13315050698228267No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14680.13242882401760908No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14590.13161693068235125No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14490.1307148269765092No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14280.12882040919424098No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13560.1223252625121784No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT13530.1220546314004258No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13520.12196442102984158No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12960.11691264027712626No Hit
CTTTTGCAGGGCCAGTCAGAGTGTTAACCGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT12930.11664200916537365No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12660.11420632915960019No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12580.11348464619492657No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCCCCCCGGAGGCCAGTTATTATA12580.11348464619492657No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12380.11168043878324252No Hit
TCCATGGTGCATCCACCAGGGCCACTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGT12200.11005665211272689No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12200.11005665211272689No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12170.10978602100097426No Hit
GTCAGAGTGTTAACCGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTG12160.10969581063039006No Hit
GAGTGTTAACCGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATC12140.10951538988922167No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12030.10852307581279545No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12030.10852307581279545No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12000.10825244470104284No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11970.10798181358929021No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11940.10771118247753762No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATCCACCAGGGCCACTGGTATCCCAGGCAGGTT11910.10744055136578501No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11910.10744055136578501No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11870.10707970988344821No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11850.1068992891422798No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11740.10590697506585357No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11570.10437339876592214No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11230.10130624616605925No Hit
GTCTGGGGCTGAGGTGAAGAATCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAG11120.10031393208963302No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11090.1000433009778804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA150.001000025785.623569
CTAACGC309.747389E-656.864083
CGTATGC2400.054.7094941
GCCGTCT2400.053.5954546
TCGTATG2450.053.5318340
GACGGAC200.007692475351.1730547
GCGGCAT400.00914325448.98118670
CGAGACG2650.047.64782323
GGTATCA13000.046.459871
CCGTCTT2800.045.99181447
GGAGCTA2800.045.1972429
ACGAGAC2800.045.09322722
ATGCCGT2850.045.0006244
ATCTCGT2700.044.58362637
CATCTCG2850.043.40244336
ATCTCCG2950.042.7945610
CACGAGA3150.040.08106221
TCTCCGA3150.040.07744211
CCCACGA3200.039.44589619
GTATGCC3350.039.23250242
Produced by FastQC (version 0.11.5)