FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108520
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGGGGGGGACGTTCGGCCAAGGGACCAAGGTGG46330.41794464691660954No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40900.36896041568938764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33530.3024753725688305No Hit
GTTATTATACTGCTGACAGTAATAAACTGCTAAATCTTCAGACTGCAGGCTGCTGATGGTGAGACTGAACT30890.2786598347346011No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29600.26702269692923897No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28420.25637787320030314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27650.24943167466531954No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGGGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA26000.23454696351892612No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25090.22633781979576373No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24930.22489445386641652No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23030.20775448345541803No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22170.19999639158517662No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCAGCCTGCAGTCTGAAGATT21670.1954858730559665No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21370.19277956193844042No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21030.18971240933857758No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20840.1879984122974777No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20580.18565294266228846No Hit
CTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGT19810.17870674412730486No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19690.17762421968029446No Hit
GTAATAAACTGCTAAATCTTCAGACTGCAGGCTGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA19020.1715801248511529No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18460.16652834409843756No Hit
GTGTATTACTGTGCGAGAGATCTGGCGGTGACTACAGTCTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGT18180.1640024537220799No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17580.15858983148702774No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17490.15777793815176994No Hit
GAGTTCAGTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTAGCAGTTTATTACTGTCAGCAGTATAATAACTGG17330.1563345722224227No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTTTGCAGGGCCAGTCAGAGTGTTAACCGCAACTTAGCCTGGTA17090.15416952332840184No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGTGGCCCTGGT16540.1492079529462707No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16500.1488471114639339No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16290.14695269368166564No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTTTTGCAGGGCCAGTCAGAGTGTTAACCG16280.14686248331108145No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16120.14541911738173421No Hit
GTAATACACAGCCGTATCTTCAAATCTCAGGCTCCTCAGCTCCATGTAGG15590.14063796774077147No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14970.13504492476455093No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14910.1345036625410457No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14800.13351134846461948No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCCCCCCGGAGG14670.13233861364702487No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG14550.13125608920001441No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATGGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG13870.12512178400028867No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13850.12494136325912027No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13740.12394904918269405No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13660.12322736621802043No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13210.1191678995417313No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12830.11573990545953163No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCCCCCCGGAGGCCAGTTATTATA12720.1147475913831054No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12340.11131959730090572No Hit
CTTTTGCAGGGCCAGTCAGAGTGTTAACCGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT12230.11032728322447947No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12190.10996644174214268No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12000.10825244470104284No Hit
GTGTTAACCGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATCCA11580.10446360913650633No Hit
GAGTGTTAACCGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATC11560.10428318839533794No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATCCACCAGGGCCACTGGTATCCCAGGCAGGTT11450.1032908743189117No Hit
GTCAGAGTGTTAACCGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTG11250.10148666690722767No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11230.10130624616605925No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11170.10076498394255404No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11140.10049435283080142No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11100.10013351134846463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.042.1231881
GTACGGT400.00275558834.0835461
CAACGCA41650.031.40275
GTATCAA43350.031.2137681
AACGCAG42100.031.0670416
CGACCGT450.004777949330.46013832
GACCGTG900.00533456429.77886470
CGCAGAG44950.029.4761478
ACGCAGA44750.029.3795457
AGAGTAC45650.028.72311611
AATGGTA4200.028.7153370
GACAGGT2500.028.630181
CGTATCT7950.028.2702513
CCGTATC8100.028.16967212
ATCAACG47650.028.0918563
TATCAAC47700.028.0700072
TCAACGC47850.028.043094
GCCGTAT8050.027.92284411
CGCGGAT8200.027.63098558
TTCGATC500.00775437927.58567439
Produced by FastQC (version 0.11.5)