FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885807
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41820.4721118708702911No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40110.4528074399953941No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29840.33686796333738617No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27880.31474124724686076No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26980.3045810204705991No Hit
AGTCAGAACATTGCCACTTCTTTAATTTGGTATCAGCAGAAGGCAGGGAT25830.2915985084787093No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24640.27816443085231884No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23980.2707135978830603No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGCCAC23970.2706007064744352No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23620.26664950717255564No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23380.26394011336555256No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22460.25355410377204063No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21550.2432809855871539No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21430.24192628868365232No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20970.23673328388689635No Hit
GTCTTACCATCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGA20290.22905666810038755No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19890.22454101175538235No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18880.21313897948424432No Hit
GTCTCCACCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGCCA17980.20297875270798268No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17750.2003822503096047No Hit
GATCTACGGTGCTTCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCA17520.19778574791122672No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17320.19552791973872413No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17010.19202828607134512No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16580.18717395550046453No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCTACTTACTATTGTCAACAGAGTCTTACCATC16560.1869481726832143No Hit
TGGTAAGACTCTGTTGACAATAGTAAGTAGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT16480.18604504141421324No Hit
GAGTCTTACCATCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT15920.179723122531206No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15730.17757818576732853No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC15040.16978867857219462No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14900.1682081988514428No Hit
GTCCTGATCTACGGTGCTTCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGA14730.1662890449048156No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14280.16120893151668478No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14160.15985423461318324No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13820.15601592671992884No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13670.15432255559055189No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13620.15375809854742625No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCA13570.1531936415043006No Hit
CCTGAAGACTTTGCTACTTACTATTGTCAACAGAGTCTTACCATCCCCCT13480.15217761882667444No Hit
CCTTAGGGGCTATCCCTGCCTTCTGCTGATACCAAATTAAAGAAGTGGCA13340.15059713910592262No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13300.1501455734714221No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13010.14687172262129336No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12940.14608148276091745No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12740.14382365458841484No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGGTGTGCATTTGCAGATACA12500.14111426078141176No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12410.14009823810378558No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12390.13987245528653533No Hit
GTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12330.13919510683478456No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGAC12180.1375017357054076No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12180.1375017357054076No Hit
CCGTAGATCAGGACCTTAGGGGCTATCCCTGCCTTCTGCTGATACCAAAT12130.13693727866228195No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12020.13569547316740554No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11960.13501812471565477No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11940.13479234189840453No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11930.1346794504897794No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT11760.1327602965431522No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11720.13230873090865164No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11720.13230873090865164No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11610.13106692541377524No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11600.13095403400515013No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC11590.130841142596525No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11480.12959933710164856No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11380.12847042301539724No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11230.12677705188602031No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11220.12666416047739518No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACT11170.12609970343426954No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10990.1240676580790172No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10940.12350320103589156No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10860.12260006976689054No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10850.1224871783582654No Hit
CCCCTAAGGTCCTGATCTACGGTGCTTCCAGTTTGCAAAGTGGGGTCCCG10620.11989067595988742No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10610.1197777845512623No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCACT10570.11932621891676179No Hit
GTCTGCAACCTGAAGACTTTGCTACTTACTATTGTCAACAGAGTCTTACCATCCCCCTCACTTTCGGCGGAGGGA10520.11876176187363614No Hit
GTTCAGTGGCAGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCTACTTA10390.11729417356150945No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10130.1143589969372561No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10110.11413321412000582No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGCCACTTCTTTAATTTGGTATCAGCAGAAGGCAGGGATAG9960.1124398429906289No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9920.11198827735612836No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9850.11119803749575247No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT9830.1109722546785022No Hit
ACTTACTATTGTCAACAGAGTCTTACCATCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA9770.11029490622675142No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9680.10927888354912527No Hit
GATCAGGACCTTAGGGGCTATCCCTGCCTTCTGCTGATACCAAATTAAAG9450.1066823811507473No Hit
GCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGGGG9420.1063437069248719No Hit
GTAAGACTCTGTTGACAATAGTAAGTAGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTC9400.10611792410762165No Hit
GACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCT9260.10453744438686982No Hit
GTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAAACT9250.1044245529782447No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9150.1032956388919934No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9120.102956964666118No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9100.10273118184886776No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC9040.10205383339711699No Hit
GTGTATTACTGTGCGAGAGATGCTTACCCCAGTCTCCTTTGTAGTCGTGA9040.10205383339711699No Hit
GGCAGGGATAGCCCCTAAGGTCCTGATCTACGGTGCTTCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGG9020.10182805057986671No Hit
GTTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCCCCTACAGATGCAGACAGGGAGGGTGGAGACTGGGTCAT8890.10036046226774005No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT8860.10002178804186465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCC451.9022591E-645.8533837
TAACGGC451.9157324E-645.8064136
GTGGTAT2100.043.8164021
TCGTATG1600.038.84811440
CGTATGC1700.038.64504641
GCTAATC1600.038.5329433
CGGTCCG4300.038.0962470
CGGTACC557.0234837E-637.91815645
CGCGGTA557.1577742E-637.81365243
TTAACGG557.68401E-637.4247335
GTATCAA23650.037.3216551
CGAGACT2050.036.599923
ATCTCGT1600.036.53940637
ATCTCCG2000.035.79147710
ACGAGAC2100.035.7224222
AATCTCG1650.035.3958636
CTAATCT1650.035.3094634
CACGAGA2150.034.88575421
CCCACGA2100.034.08712419
GCCCACG2100.034.0813518
Produced by FastQC (version 0.11.5)