FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885807
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41620.4698540426977886No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31960.36080094196591356No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29220.3298686960026281No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26060.2941950108770872No Hit
AGTCAGAACATTGCCACTTCTTTAATTTGGTATCAGCAGAAGGCAGGGAT25150.2839218926922005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25130.2836961098749502No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23500.2652948102690541No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22970.25931156561192226No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22070.2491513388356606No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22060.2490384474270355No Hit
GTCTTACCATCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGA21110.2383137636076482No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21050.2376364151558974No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGCCAC21000.23707195811277174No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19700.22239607499150493No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19380.21878354991550075No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19010.214606567796371No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18620.21020380285999093No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18580.2097522372254904No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18100.20433344961148422No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCTACTTACTATTGTCAACAGAGTCTTACCATC17940.20252718707348213No Hit
TGGTAAGACTCTGTTGACAATAGTAAGTAGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT17020.19214117747997025No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16970.1915767204368446No Hit
GTCTCCACCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGCCA16920.19101226339371893No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16600.1873997383177148No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16560.1869481726832143No Hit
GATCTACGGTGCTTCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCA16330.18435167028483632No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15950.1800617967570814No Hit
GAGTCTTACCATCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT15750.1778039685845788No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15550.17554614041207622No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15160.17114337547569616No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCA14890.16809530744281767No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14780.16685350194794124No Hit
GTCCTGATCTACGGTGCTTCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGA14230.16064447447355915No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13780.15556436108542832No Hit
CCTTAGGGGCTATCCCTGCCTTCTGCTGATACCAAATTAAAGAAGTGGCA13550.15296785868705035No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCACT13510.15251629305254982No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13410.15138737896629853No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13390.15116159614904828No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGAC13340.15059713910592262No Hit
CCTGAAGACTTTGCTACTTACTATTGTCAACAGAGTCTTACCATCCCCCT13040.14721039684716875No Hit
GTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13030.1470975054385436No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12990.14664593980404309No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGGTGTGCATTTGCAGATACA12650.14280763191078868No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12520.141340043598662No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12250.13829197556578351No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12140.13705017007090708No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12100.1365986044364066No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12060.13614703880190604No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC12020.13569547316740554No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12010.1355825817587804No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11850.13377631922077834No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11770.13287318795177733No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11700.1320829480914014No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11520.13005090273614908No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11150.1258739206170193No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11110.12542235498251875No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11020.1244063323048926No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10970.12384187526176696No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10860.12260006976689054No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10850.1224871783582654No Hit
CCGTAGATCAGGACCTTAGGGGCTATCCCTGCCTTCTGCTGATACCAAAT10740.12124537286338899No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACT10690.12068091582026334No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10600.11966489314263716No Hit
CCCCTAAGGTCCTGATCTACGGTGCTTCCAGTTTGCAAAGTGGGGTCCCG10560.11921332750813665No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC10500.11853597905638587No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGCCACTTCTTTAATTTGGTATCAGCAGAAGGCAGGGATAG10390.11729417356150945No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10370.11706839074425918No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10240.11560080243213251No Hit
GTTCAGTGGCAGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCTACTTA10170.1148105625717566No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10140.1144718883458812No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10050.11345586566825505No Hit
GTCTGCAACCTGAAGACTTTGCTACTTACTATTGTCAACAGAGTCTTACCATCCCCCTCACTTTCGGCGGAGGGA10030.11323008285100478No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT10010.11300430003375454No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9910.11187538594750322No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9820.11085936326987707No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9710.10961755777500065No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9520.10747262101112318No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9430.10645659833349702No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9410.10623081551624676No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9390.10600503269899651No Hit
GTAAGACTCTGTTGACAATAGTAAGTAGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTC9380.10589214129037139No Hit
ACTTACTATTGTCAACAGAGTCTTACCATCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA9380.10589214129037139No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9360.10566635847312111No Hit
GTTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCCCCTACAGATGCAGACAGGGAGGGTGGAGACTGGGTCAT9310.10510190142999547No Hit
GTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCACTTTGCAAACT9280.1047632272041201No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9180.10363431311786878No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9000.10160226776261647No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9000.10160226776261647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACG150.00227745569.5876745
CGAATAG200.006556831353.28152558
GCGAATA200.00661829153.15599457
ATCGGAG306.279865E-446.0456140
TCGATAC350.001391396939.1731234
CGGTCCG2800.035.20646770
GTATCAA25100.034.929921
GTGGTAT2550.034.6485251
TACGGAT502.0387204E-433.9687777
ACCCATA552.2844099E-433.31662866
GATCGGA450.004637307530.64606339
TATCAAC28950.030.2795332
ATCAACG29250.029.8477573
TCTTCGG2651.2732926E-1129.75942670
ACAGGTT1600.029.7344321
TCAACGC29600.029.2636454
GCTTATT900.005862408329.20832870
AACGCAG29900.029.0836336
CAACGCA29950.029.0350825
CGTTTGG500.00704273528.13444153
Produced by FastQC (version 0.11.5)