FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598408
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGCTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC39190.6549043461985802No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35810.5984211441023516No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31760.5307415676261013No Hit
AGCCAGGACTCTGCATAACTTGCATGCAGTAATAAACCCCAACATCCTCA26150.43699282095159153No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25360.4237911257870884No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23140.3866926912741808No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20220.3378965521851312No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19710.3293739388510849No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19420.3245277469552546No Hit
GGTCTAGTCAGAGCCTCCTGCATCCTAATGGATACAACTTTTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTC19150.32001577519017127No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18860.31516958329434097No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18770.3136655927059799No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGTTGATTCTCAGCGTAAAATCTGTGCCTGATCCACTGCCA18030.301299447868344No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT17820.2977901364955014No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17790.29728880629938104No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17150.28659376211547977No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17060.2850897715271186No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16880.2820817903503964No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16540.2764000481276988No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15800.26403390329006293No Hit
CCCCTGGTGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG15750.26319835296319566No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15130.2528375289100413No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACGCTGAG14830.2478242269488376No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14750.2464873464258499No Hit
GTCTCCACTCTCCCTGGCCGTCACCCCTGGTGAGCCGGCCTCCATCTCCT14330.23946872368016472No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14150.23646074250344246No Hit
CTGCATAACTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTC14130.23612652237269555No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC14050.23478964184970788No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTTATCGGGCC13390.2237603775350597No Hit
GTTATGCAGAGTCCTGGCTTTGGCCAGGGGACCAAGGTGGACATCAAACG13280.22192216681595164No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGA12620.21089290250130344No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12600.21055868237055655No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTCCACTCTCCCTGGCCGTCACCC12380.20688226093234047No Hit
GTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTTA12230.2043756099517386No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12130.20270450929800402No Hit
GATTTTACGCTGAGAATCAACAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTATGCAGAGT11890.19869386772904105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11680.19518455635619844No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTCCACTCTCC11650.19468322616007808No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11550.1930121255063435No Hit
GTTTATTACTGCATGCAAGTTATGCAGAGTCCTGGCTTTGGCCAGGGGAC11540.19284501544097005No Hit
GGTATGTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGT11520.1925107953102231No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11520.1925107953102231No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11510.19234368524484968No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCAAAGCCA11490.19200946511410277No Hit
CCTTGGTCCCCTGGCCAAAGCCAGGACTCTGCATAACTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCA11430.19100680472186202No Hit
GAGATATTGTGATGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGTGAGCCGGCCTCCATCTCCTGCAGGT11410.1906725845911151No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACGCTGAGAATCAACAGAG11320.18916859400275396No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10990.18365396184542987No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10800.18047887060333417No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTCCACT10680.1784735498188527No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCACATA10660.17813932968810578No Hit
GAATCAACAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAA10500.17546556864213045No Hit
GAGAATCAACAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTATGCAGAGTCCTGGCTTTGG10480.17513134851138354No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10390.17362735792302242No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10320.1724575874654082No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCCCT10110.1689482760925656No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9930.16594029491584336No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9920.1657731848504699No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9830.1642691942621088No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9600.16042566275851927No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9540.15942300236627854No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9460.15808612184329088No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9450.1579190117779174No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9310.155579470862689No Hit
CTGTTGATTCTCAGCGTAAAATCTGTGCCTGATCCACTGCCACTGAACCT9270.15491103060119518No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9200.15374126014358097No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9170.1532399299474606No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9100.1520701594898464No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8950.14956350850924455No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGTTGATTCTCAGCGTAAAATCTGTGCCTGATCCACTGCCA8940.14939639844387106No Hit
AGTCAGAGCCTCCTGCATCCTAATGGATACAACTTTTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTCCACAA8930.1492292883784976No Hit
GCGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG8830.14755818772476303No Hit
TTATTACTGCATGCAAGTTATGCAGAGTCCTGGCTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGG8830.14755818772476303No Hit
CTCCCTGGCCGTCACCCCTGGTGAGCCGGCCTCCATCTCCTGCAGGTCTA8720.145719977005655No Hit
CTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTT8690.14521864680953464No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8670.1448844266787877No Hit
CCACTCTCCCTGGCCGTCACCCCTGGTGAGCCGGCCTCCATCTCCTGCAG8580.1433804360904266No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTATGCAG8530.1425448857635593No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8510.1422106656328124No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8500.14204355556743894No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8430.14087378510982473No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCACCAGGGGTGACGGCCAGG8430.14087378510982473No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8410.14053956497907782No Hit
GCCCTGGCTTCTGCACATACCAATCCAAAAAGTTGTATCCATTAGGATGC8250.1378658039331025No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8200.13703025360623522No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8150.13619470327936792No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA8080.1350249328217537No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8030.13418938249488643No Hit
ATTTGGGTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT7910.13218406171040495No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7800.1303458509912969No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT7770.12984452079517653No Hit
GTTGATTCTCAGCGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGT7710.1288418604029358No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7700.12867475033756232No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7660.1280063100760685No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7600.12700364968382777No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7580.12666942955308083No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTC7540.126000989291587No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7530.12583387922621356No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7440.12432988863785242No Hit
GCACATACCAATCCAAAAAGTTGTATCCATTAGGATGCAGGAGGCTCTGA7430.12416277857247898No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCACATACCAATCCAAAAAGTTGTATCCATTAGGATGCAGGAGGCTCTGA7430.12416277857247898No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7280.12165612759187712No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7240.12098768733038329No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7150.11948369674202218No Hit
GGCACAGATTTTACGCTGAGAATCAACAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTATG6940.11597438536917956No Hit
CCACAACTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCCCTGA6840.11430328471544499No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT6810.11380195451932461No Hit
GGCCAAAGCCAGGACTCTGCATAACTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGTTGATT6800.11363484445395114No Hit
CCCTGGCCGTCACCCCTGGTGAGCCGGCCTCCATCTCCTGCAGGTCTAGT6710.11213085386559003No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6700.11196374380021658No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6700.11196374380021658No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6650.11112819347334929No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6610.11045975321185547No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG6590.11012553308110853No Hit
CTTCTGCACATACCAATCCAAAAAGTTGTATCCATTAGGATGCAGGAGGC6550.10945709281961472No Hit
GATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCACATACCAATCCAAAA6550.10945709281961472No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT6480.10828732236200052No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA6290.10511223111990481No Hit
CACCAGGGGTGACGGCCAGGGAGAGTGGAGACTGAGTCATCACAATATCT6190.10344113046617023No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6170.10310691033542332No Hit
GGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGTGAGCCGG6150.10277269020467641No Hit
GCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCAAAGCCAGGACT6150.10277269020467641No Hit
GGTTTATTACTGCATGCAAGTTATGCAGAGTCCTGGCTTTGGCCAGGGGA6140.10260558013930295No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6110.10210424994318258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGT200.005914461354.68602457
GTGCCGC200.006553546553.28593449
TGTCGTA601.0186341E-1051.46505438
TAACGCT200.00836731850.0930524
TATACTG200.00836731850.0930525
TGCTTGA3300.048.31009355
CTAACGC701.0913936E-1147.7076723
TCGTATG3350.047.3930640
CGTATGC3500.046.5127741
ATGCCGT3500.045.969444
GTCGTAT605.7370926E-945.87669839
GTATGCC3650.045.68885442
GCTTGAA3500.045.66544756
GCCGTCT3450.044.83165746
GTGTATA307.403017E-444.5308841
CCGTCTT3500.044.33159347
ATCTCGT2800.043.95816437
AATCTCG2800.043.8643436
CGTCTTC3350.043.31919548
TCCGAGC3650.043.0058513
Produced by FastQC (version 0.11.5)