FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598408
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40280.6731193433242871No Hit
GTCCTGGCTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC37600.6283338458042005No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36600.6116228392668548No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26540.4435101135011564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25870.43231373912113474No Hit
AGCCAGGACTCTGCATAACTTGCATGCAGTAATAAACCCCAACATCCTCA24690.41259475140706675No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21210.35444044865710356No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20030.33472146094303556No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19390.32402641675913424No Hit
GGTCTAGTCAGAGCCTCCTGCATCCTAATGGATACAACTTTTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTC19330.3230237563668935No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18550.30998917126776376No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGTTGATTCTCAGCGTAAAATCTGTGCCTGATCCACTGCCA18260.3051429793719336No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17290.28893330303070813No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16470.2752302776700846No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16460.27506316760471117No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16310.2725565166241093No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15790.26386679322468953No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15600.2606917019825938No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15170.2535059691715351No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14840.24799133701421103No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14770.24682156655659684No Hit
GTCTCCACTCTCCCTGGCCGTCACCCCTGGTGAGCCGGCCTCCATCTCCT14640.24464913570674188No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14610.2441478055106215No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14310.2391345035494178No Hit
CTGCATAACTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTC13990.23378698145746715No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13980.2336198713920937No Hit
CCCCTGGTGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG13680.22860656943088997No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC13260.22158794668520476No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACGCTGAG13060.2182457453777356No Hit
GTTATGCAGAGTCCTGGCTTTGGCCAGGGGACCAAGGTGGACATCAAACG12850.21473643400489298No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12680.2118955628935442No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTTATCGGGCC12660.2115613427627973No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12310.20571249047472628No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTCCACTCTCCCTGGCCGTCACCC12070.20170184890576331No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTCCACTCTCC11820.19752409727142686No Hit
GGTATGTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGT11720.1958529966176923No Hit
GTTTATTACTGCATGCAAGTTATGCAGAGTCCTGGCTTTGGCCAGGGGAC11640.19451611609470462No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACGCTGAGAATCAACAGAG11460.19150813491798238No Hit
GTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTTA11440.19117391478723547No Hit
GATTTTACGCTGAGAATCAACAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTATGCAGAGT11410.1906725845911151No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTCCACT11400.19050547452574162No Hit
CCTTGGTCCCCTGGCCAAAGCCAGGACTCTGCATAACTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCA11380.19017125439499472No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11240.1878317134797663No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCCTGGCCAAAGCCA11080.185157952433791No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGA10900.18214997125706878No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10740.17947621021109345No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10720.17914199008034654No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10580.17680244916511811No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10270.17162203713854093No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCACATA10230.17095359687704711No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCCCT10180.1701180465501798No Hit
GAATCAACAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAA10150.16961671635405945No Hit
GCGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG10100.16878116602719215No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10090.1686140559618187No Hit
GAGATATTGTGATGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGTGAGCCGGCCTCCATCTCCTGCAGGT10030.16761139556957794No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9870.16493763452360263No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9860.16477052445822918No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9820.16410208419673533No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9700.16209676341225385No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9590.16025855269314582No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9520.1590887822355316No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9470.1582532319086643No Hit
CTCCCTGGCCGTCACCCCTGGTGAGCCGGCCTCCATCTCCTGCAGGTCTA9440.15775190171254394No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTATGCAG9340.15608080105880937No Hit
GAGAATCAACAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTATGCAGAGTCCTGGCTTTGG9240.1544097004050748No Hit
CTGTTGATTCTCAGCGTAAAATCTGTGCCTGATCCACTGCCACTGAACCT9140.15273859975134021No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8980.15006483870536488No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGTTGATTCTCAGCGTAAAATCTGTGCCTGATCCACTGCCA8880.1483937380516303No Hit
AGTCAGAGCCTCCTGCATCCTAATGGATACAACTTTTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTCCACAA8770.1465555273325223No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8710.14555286694028155No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8630.14421598641729388No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8560.1430462159596797No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8520.14237777569818585No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA8490.14187644550206546No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8480.14170933543669204No Hit
CTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTT8450.14120800524057164No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8330.13920268445609016No Hit
GCCCTGGCTTCTGCACATACCAATCCAAAAAGTTGTATCCATTAGGATGC8290.1385342441945963No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8250.1378658039331025No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8250.1378658039331025No Hit
TTATTACTGCATGCAAGTTATGCAGAGTCCTGGCTTTGGCCAGGGGACCAAGGTGGACATCAAACGAACTGTGG8210.13719736367160867No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT8190.13686314354086176No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7980.13335383216801913No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7940.1326853919065253No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCACCAGGGGTGACGGCCAGG7930.13251828184115186No Hit
CCACTCTCCCTGGCCGTCACCCCTGGTGAGCCGGCCTCCATCTCCTGCAG7710.1288418604029358No Hit
GTTGATTCTCAGCGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGT7670.12817342014144195No Hit
ATTTGGGTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT7530.12583387922621356No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7520.1256667691608401No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7490.1251654389647197No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7420.12399566850710551No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7370.12316011818023823No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCACATACCAATCCAAAAAGTTGTATCCATTAGGATGCAGGAGGCTCTGA7370.12316011818023823No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7330.12249167791874441No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7310.12215745778799748No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7260.1213219074611302No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7160.11965080680739562No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7120.1189823665459018No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7010.11714415582679376No Hit
GGCACAGATTTTACGCTGAGAATCAACAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTATG6940.11597438536917956No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC6870.11480461491156536No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6870.11480461491156536No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT6710.11213085386559003No Hit
GGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGTGAGCCGG6630.11079397334260238No Hit
GCACATACCAATCCAAAAAGTTGTATCCATTAGGATGCAGGAGGCTCTGA6620.11062686327722891No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6620.11062686327722891No Hit
GGCCAAAGCCAGGACTCTGCATAACTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGTTGATT6590.11012553308110853No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6480.10828732236200052No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGCGGAGATATTGTGATGACTCAGTCTC6470.10812021229662705No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG6380.10661622170826594No Hit
CCACAACTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCCCTGA6350.10611489151214556No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6340.1059477814467721No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT6230.10410957072766405No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG6230.10410957072766405No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6180.10327402040079678No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT6170.10310691033542332No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6130.10243847007392949No Hit
GGTTTATTACTGCATGCAAGTTATGCAGAGTCCTGGCTTTGGCCAGGGGA6100.10193713987780911No Hit
GATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCACATACCAATCCAAAA6060.1012686996163153No Hit
CTTCTGCACATACCAATCCAAAAAGTTGTATCCATTAGGATGCAGGAGGC6020.10060025935482148No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6010.100433149289448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)