FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110697
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGTTTATTCTCGCACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT33630.30278284716713916No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31650.28495620317692405No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31280.281624961623197No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26390.23759855298069588No Hit
ACATTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC25080.22580415720939193No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATACA23310.20986821788480567No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21890.1970834530029342No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21760.19591301678135442No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21720.19555288255932987No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA21100.18997080211794937No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTA20860.18780999678580207No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20790.1871797618972591No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20790.1871797618972591No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20320.18294818478847066No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19460.17520529901494286No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18860.16980328568457465No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18810.16935311790704397No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG18340.1651215407982555No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18180.1636810039101573No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17930.16143016502250387No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16880.1519766416943595No Hit
GAGTATTGATAATTCGTTGGCCTGGTATCAGCACAAACCAGGGAAAGCCCCTAAACTCCTGATCTTTCATGCCTC16540.14891550080715082No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16480.14837529947411401No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGTTCTGG16460.14819523236310173No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15450.13910184325698186No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15180.1366709372583162No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15040.13541046748123026No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15030.13532043392572413No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14090.12685727970814722No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14080.1267672461526411No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13670.12307587037688947No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13400.12064496437822377No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13250.11929446104563171No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13060.11758382349101511No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12930.11641338726943531No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12840.11560308526988008No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12570.11317217927121438No Hit
GCACAAACCAGGGAAAGCCCCTAAACTCCTGATCTTTCATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT12540.11290207860469596No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12530.11281204504918983No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGTTCTGGCTCCCAGGTGCCAAATGTGACAT12510.11263197793817757No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12350.11119144105007936No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTG12310.11083130682805482No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT12240.11020107193951187No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12200.1098409377174873No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACTATCTCCAGAGACAACGCCCAGAACTCTCTGTATCTCCAAATGAA12170.1095708370509689No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATA12160.10948080349546276No Hit
GCTCCTGGGGCTCCTGCTGTTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC11850.10668976327477252No Hit
GTATCAGCACAAACCAGGGAAAGCCCCTAAACTCCTGATCTTTCATGCCT11780.10605952838622955No Hit
ACTTATTACTGCCAACAGTATAATGTTTATTCTCGCACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11560.10407879016509454No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11500.1035385888320577No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT11280.10155785061092268No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11240.10119771638889814No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11200.10083758216687361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA150.002372224168.8770940
GTCGTAT309.313408E-657.30275339
GATCCGT200.007163599652.1032744
AATCGCG200.007656384351.23393236
ATGTCGT352.3889095E-548.872237
ATATCGT306.578862E-445.61404837
TCGTATG2500.045.45887840
CGTATGC2450.043.6656241
GTGGTAT3150.042.8337021
GACGTAG3550.041.8986526
CGTAGAG3600.041.37833428
GAGACGT3600.041.2776224
ACGAGAC3800.040.86419322
ACGTAGA3750.040.59481427
CGAGACG3750.040.51798223
CACGAGA3950.040.1561521
TACGTTA350.00126614839.93284647
AGACGTA3750.039.64080425
ATCTCGT1850.038.83358437
CGAGCCC4100.038.66612215
Produced by FastQC (version 0.11.5)