FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110697
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35630.32078955826836664No Hit
GTATAATGTTTATTCTCGCACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32580.2933293238389948No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31420.2828854314002829No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24730.22265298276667717No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24140.21734100299181505No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23560.2121190567724591No Hit
ACATTATACTGTTGGCAGTAATAAGTCGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC23440.21103865410638545No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATACA22360.2013150301117226No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21320.19195154033908438No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19230.17313452723830172No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTA18930.1704335205731176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18780.16908301724052555No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18630.16773251390793348No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18460.16620194346432915No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17800.16025972880092412No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17600.15845905769080137No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16410.14774506458557105No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16350.1472048632525342No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16100.1449540243648808No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16090.14486399080937465No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15480.13937194392350027No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15120.13613073592527936No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14540.1309087897059234No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG14480.13036858837288656No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14420.12982838703984975No Hit
GAGTATTGATAATTCGTTGGCCTGGTATCAGCACAAACCAGGGAAAGCCCCTAAACTCCTGATCTTTCATGCCTC14120.12712738037466564No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14030.12631707837511041No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13560.12208550126632195No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13540.12190543415530967No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13420.12082503148923604No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13400.12064496437822377No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGTTCTGG13340.12010476304518693No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12990.11695358860247214No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12520.11272201149368369No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12260.11038113905052413No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12040.10840040082938912No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTG11910.10722996460780933No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11910.10722996460780933No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11640.10479905860914362No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11510.10362862238756385No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGATAATTCGTTGGCCTGGTATCAGCACAAA11490.10344855527655158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTCG200.0080963950.5148287
GTGGTAT3200.043.194761
AAGACGA450.001302880739.70022669
GTATCAA25750.034.302141
GGTCCGA450.00395603731.65809654
TCTGGAG9650.031.34899770
TACCCGT900.004201696731.26798870
TATCAAC28900.030.2079182
ATCAACG30250.029.0682413
CTGTCCG550.00606370929.01032668
TCAACGC30600.028.836774
CAACGCA31100.028.3705985
AACGCAG31900.027.8702496
CAGGTAT1504.7293724E-1126.9703851
GTAACAC500.00868501826.9521643
TAGATTA658.0569094E-426.8667746
ACAGACG7200.026.38236470
ACGCAGA33600.026.3599197
CGCAGAG33700.026.2816988
CCTCGTG908.33386E-626.1916929
Produced by FastQC (version 0.11.5)