FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044963
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGGGGCGCTCACAGAGGTCAACTTCAGGGAGA59830.5725561574907437No Hit
GTATGGAACCGTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT41800.4000141631808973No Hit
GTATTACTGTCAGCAGTATGGAACCGTACCGTGGACGTTCGGCCAAGGGA37960.3632664505824608No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35350.338289489675711No Hit
GTTCCATACTGCTGACAGTAATACACTGCAGAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTC34730.3323562652457551No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30770.29446018662861745No Hit
GTCTCTGGTGGCGCCATCAGTAGTAGCATTTATTACTGGGGCTGGATCCG27260.2608704805816091No Hit
GTATATTACTGTGCGGGGGGGGCTACGATTGACGGGGATGTGACCTATTA26730.2557985306656791No Hit
GTGTATTACTGTCAGCAGTATGGAACCGTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAA22770.21790245204854142No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22550.21579711434758933No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21990.21043807292698402No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21950.21005528425408365No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21930.20986388991763344No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21360.20440915132880302No Hit
GTATATTACTGTGCGGGGGGGGCTACGATTGACGGGGATGTGACCTATTACGCCATGGACGTCTGGGGCCAAGGG20850.19952859574932316No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20350.19474373733806843No Hit
CACCTGCACTGTCTCTGGTGGCGCCATCAGTAGTAGCATTTATTACTGGG19140.18316437998283192No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18690.17885800741270264No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCG18580.1778053385622266No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18370.17579569802949963No Hit
GTCTATGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCACG18340.17550860652482433No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18240.17455163484257338No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17830.17062805094534447No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17580.16823562173971712No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17390.1664173755434403No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17250.16507761518828895No Hit
GAACCGTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGA16830.16105833412283496No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16580.15866590491720758No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16430.15723044739383116No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16420.15713475022560608No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16370.15665626438448058No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16070.15378534933772775No Hit
CTCCTGCAGGGCCAGTCACGGTGTTCCCGTCAACTACTTAGCCTGGTACC16070.15378534933772775No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTAGAGCCTGAAGATT16000.1531154691601521No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15860.15177570880500077No Hit
GTAATACACTGCAGAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15550.1488090965900228No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15070.14421563251521824No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15010.14364144950586769No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGACGGGAACACCGTGA14350.1373254364030114No Hit
GTACGGTTCCATACTGCTGACAGTAATACACTGCAGAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGT14280.13665555622543574No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14110.13502870436560913No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14090.1348373100291589No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13710.13120081763660532No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13650.13062663462725474No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13550.1296696629450038No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13510.12928687427210342No Hit
CAGTAATATACAGCCGTGTCTGGGGCGCTCACAGAGGTCAACTTCAGGGA13130.1256503818795498No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13010.12450201586084866No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT12630.12086552346829504No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12450.11914297444024333No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12370.11837739709444257No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12320.1178989112533171No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12260.11732472824396653No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12210.11684624240284106No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12100.11579357355236501No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12050.11531508771123955No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12020.11502799620656426No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12010.11493229903833915No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT11970.11454951036543878No Hit
CTATATTATCATGCGAAAGGCTACTACAACCCGTCCCTCCAGAGTCGAGT11720.11215708115981139No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11330.1084248915990327No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11220.10737222274855665No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC11200.10718082841210647No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11170.10689373690743117No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11040.10564967372050493No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11020.10545827938405475No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10770.10306585017842737No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10660.10201318132795133No Hit
GTCTCCTGACACCCTGTCTATGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCACGGTGTTCCCGT10650.10191748415972622No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10630.10172608982327604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)