FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044963
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGGGGCGCTCACAGAGGTCAACTTCAGGGAGA61140.5850924865282312No Hit
GTATGGAACCGTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT40900.3914014180406387No Hit
GTATTACTGTCAGCAGTATGGAACCGTACCGTGGACGTTCGGCCAAGGGA36180.34623235463839386No Hit
GTTCCATACTGCTGACAGTAATACACTGCAGAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTC35240.33723682082523493No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34040.3257531606382235No Hit
GTATATTACTGTGCGGGGGGGGCTACGATTGACGGGGATGTGACCTATTA27190.2602006004040335No Hit
GTCTCTGGTGGCGCCATCAGTAGTAGCATTTATTACTGGGGCTGGATCCG25800.24689869402074524No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25130.24048698374966385No Hit
GTGTATTACTGTCAGCAGTATGGAACCGTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAA23110.22115615576819464No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22760.2178067548803163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22200.212447713459711No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22050.21101225593633458No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20210.1934039769829171No Hit
GTATATTACTGTGCGGGGGGGGCTACGATTGACGGGGATGTGACCTATTACGCCATGGACGTCTGGGGCCAAGGG18990.18172892245945552No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18720.17914509891737793No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18160.17378605749677262No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCG18060.17282908581452167No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18050.17273338864629656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17960.17187211413227071No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17960.17187211413227071No Hit
GTCTATGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCACG17860.17091514245001976No Hit
CACCTGCACTGTCTCTGGTGGCGCCATCAGTAGTAGCATTTATTACTGGG17720.16957538209486844No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17370.16622598120699011No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16460.15751753889850645No Hit
GTACGGTTCCATACTGCTGACAGTAATACACTGCAGAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGT16380.15675196155270568No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16250.15550789836577947No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTAGAGCCTGAAGATT16000.1531154691601521No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15640.14967037110404866No Hit
CTCCTGCAGGGCCAGTCACGGTGTTCCCGTCAACTACTTAGCCTGGTACC15630.14957467393582358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15360.146990850393746No Hit
GTAATACACTGCAGAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15230.14574678720681977No Hit
GAACCGTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGA15140.1448855126927939No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15100.14450272401989353No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14980.1433543580011924No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14870.14230168915071634No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14510.1388565910946129No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14330.1371340420665612No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14170.13560288737495968No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14000.1339760355151331No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13960.1335932468422327No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13950.1334975496740076No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13930.1333061553375574No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13730.1313922119730555No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13690.13100942330015514No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13340.1276600224122768No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13280.12708583940292623No Hit
CAGTAATATACAGCCGTGTCTGGGGCGCTCACAGAGGTCAACTTCAGGGA12940.12383213568327299No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12680.12134400930942052No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12610.12067412913184486No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGACGGGAACACCGTGA12420.11885588293556806No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12390.11856879143089277No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG12380.11847309426266768No Hit
CTATATTATCATGCGAAAGGCTACTACAACCCGTCCCTCCAGAGTCGAGT12380.11847309426266768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12240.11713333390751635No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12090.1156978763841399No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11620.11120010947756045No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT11580.11081732080466006No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11540.11043453213175969No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11190.10708513124388136No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC11140.10660664540275588No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT11120.1064152510663057No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11090.10612815956163042No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10990.10517118787937946No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10870.10402282186067834No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10710.10249166716907679No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10500.10048202663634981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT14300.056.95524670
TAAACCG303.6010795E-451.54224468
GGTATCA8250.044.1423531
TGGTATC2550.040.0277442
GTATCAA22200.038.4810031
GTGGTAT2700.037.8166581
CTACGGA14650.037.7444469
TACGTAT750.002050062436.19822370
ACGGTAA400.002547973334.63218342
TATCAAC26000.033.107562
ATCAACG26000.032.9640733
TCAACGC26250.032.7765584
CTAAGGG850.003786523131.9396170
CAACGCA27100.031.8755255
AACGCAG26900.031.7332366
TCTACGG15650.031.61696868
ATTACGC13500.031.23403547
TTACGCC13700.031.09037248
GTAATAT14350.031.070271
ACGTGTC14850.030.97609363
Produced by FastQC (version 0.11.5)