FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752732
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACTCAGGGTATTGTCATGGGA28840.38313769043962526No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28560.37941790703729883No Hit
GGGTATTGTCATGGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGG26970.3582948512883735No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24480.32521534888911324No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23900.31751008327000846No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTC23150.3075463777280626No Hit
CCCAGGTACAGCCCCCAAACTCCTCATCCATGGTGACGCCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG20900.27765526110222494No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18700.24842839151251708No Hit
ACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCT17410.23129081798037016No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15740.20910496697363737No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15660.20804217171582978No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15470.20551803297853685No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15290.20312674364846983No Hit
GACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACTCAGG15220.20219679779788824No Hit
GTACCTGGGAGGTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTT15200.2019310989834363No Hit
GGTTATGATGTACACTGGTACCAGCACCTCCCAGGTACAGCCCCCAAACTCCTCATCCATGGTGACGCCAATCGG15050.19993835787504716No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14760.19608572506549476No Hit
GATGTACACTGGTACCAGCACCTCCCAGGTACAGCCCCCAAACTCCTCAT14720.19555432743659099No Hit
GTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTC14580.19369443573542777No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGT14460.1921002428487164No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13980.18572347130187103No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13740.18253508552844838No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13660.1814722902706408No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTACGCTGTGCTCGTGGATGTGTCTGTGGT13630.18107374204896298No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13280.1764240127960549No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13240.1758926151671511No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13180.17509551872379545No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12540.16659315666133498No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12400.16473326496017177No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12370.16433471673849392No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12320.16367046970236418No Hit
GTCATGGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG12220.16234197563010475No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11990.159286439263908No Hit
GGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT11850.1574265475627448No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAG11330.15051837838699564No Hit
GGGCAGAGGGTCACCATCTCCTGCGCTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG11290.14998698075809186No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11200.14879133609305836No Hit
CTCCTGCGCTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTCCCAGGTACAGC10820.14374305861847245No Hit
GGATGAGGCTGATTATTTCTGCCAGTCCCATGACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAG10730.14254741395343895No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTC10480.1392261787727903No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTC10200.13550639537046386No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGC10200.13550639537046386No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9920.1317866119681374No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCCAT9820.13045811789587794No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9650.12819967797303689No Hit
GTCCCATGACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCGTAGGTCAGCCCAAGG9410.1250112921996142No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCGCTGGGAGCAG9350.12421419575625854No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9330.12394849694180665No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGCGCAGGAGA9280.12328424990567692No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8970.11916591828167263No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8940.11876737005999478No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8870.11783742420941316No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8760.11637608072992778No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCC8750.11624323132270184No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8650.11491473725044239No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8600.11425049021431266No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8600.11425049021431266No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT8590.11411764080708672No Hit
GCTTGGTCCCTCCGCCGAATACCGAAGCACTCAGGGTATTGTCATGGGACTGGCAGAAATAATCAGCCTCATCCT8520.11318769495650512No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8490.11278914673482728No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAG8450.1122577491059235No Hit
GTGTATTACTGTGCGAGATCCCGTTATAGAGATGGTGGAGTGCTTATTATCGGTGATGTTTATGATGTCTGGGGC8420.11185920088424565No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTCCCA8360.11106210444089No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8280.10999930918308243No Hit
GTATTGTCATGGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAG8250.10960076096140459No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8190.10880366451804892No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCT8140.1081394174819192No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8110.10774086926024136No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8070.10720947163133758No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8020.10654522459520785No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7960.1057481281518522No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7950.10561527874462624No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTCAGGGACC7950.10561527874462624No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7930.10534957993017435No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7820.10388823645068895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1800.045.413771
AGGGGGG1900.045.12827370
CGTATGC1450.042.78202441
GCTTGGG651.2738383E-841.92041
TCGTATG1400.041.79490740
GCCGTCT1450.040.6814546
TCTCGTA1100.040.57932338
ATCTCGT1150.038.78383337
ACTCGCT1500.038.6659229
ACGAGAC1600.038.33314522
GTCGTCG451.0797751E-437.84480712
CTAATCT1200.037.08841734
CCGTCTT1600.036.91503547
TCGCTAA1600.036.2903631
ACACTCG1600.036.23724427
CTTATAC1600.036.1890951
ATCAACG18950.035.4083563
CGCTAAT1550.035.28563332
TCAACGC19100.035.1302834
ATCTCCG1650.035.09245310
Produced by FastQC (version 0.11.5)