FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752732
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26010.3455413081946828No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23920.31777578208446033No Hit
GCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACTCAGGGTATTGTCATGGGA23660.31432169749658573No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21930.2913387500464973No Hit
GGGTATTGTCATGGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGG21070.279913701025066No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG19660.2611819346062078No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTC17980.23886323419224903No Hit
CCCAGGTACAGCCCCCAAACTCCTCATCCATGGTGACGCCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG17630.23421350493934096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16670.22145996184565025No Hit
ACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCT16050.21322329859764164No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15710.20870641875195953No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15530.20631512942189253No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15080.200336906096725No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14190.1885133088536159No Hit
GGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT13920.18492637485851537No Hit
GGTTATGATGTACACTGGTACCAGCACCTCCCAGGTACAGCCCCCAAACTCCTCATCCATGGTGACGCCAATCGG13590.1805423444200592No Hit
GATGTACACTGGTACCAGCACCTCCCAGGTACAGCCCCCAAACTCCTCAT13580.18040949501283327No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13390.1778853562755403No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13230.1757597657599252No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTACGCTGTGCTCGTGGATGTGTCTGTGGT13070.17363417524431007No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGT12680.16845304836249822No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12670.16832019895527225No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12580.16712455429023876No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12490.16592890962520526No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12070.16034923452171557No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11700.1554338064543556No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11590.15397246297487022No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC11570.1537067641604183No Hit
GTACCTGGGAGGTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTT11490.15264396890261075No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11460.1522454206809329No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC11370.15104977601589942No Hit
GACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACTCAGG11130.14786139024247674No Hit
GGATGAGGCTGATTATTTCTGCCAGTCCCATGACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAG11080.14719714320634703No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11010.1462671973557654No Hit
GGGCAGAGGGTCACCATCTCCTGCGCTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG10990.1460014985413135No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTC10910.14493870328350594No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10720.14241456454621298No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10380.13789768470053088No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10340.1373662870716271No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10110.13431075070543036No Hit
GTCATGGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG10080.13391220248375252No Hit
GTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTC10080.13391220248375252No Hit
CTCCTGCGCTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTCCCAGGTACAGC9890.13138806374645956No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAG9690.12873107560194066No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9630.127933979158585No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9460.12567553923574393No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9440.12540984042129205No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCCAT9410.1250112921996142No Hit
GTCCCATGACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCGTAGGTCAGCCCAAGG9290.12341709931290286No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9170.12182290642619152No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT9110.12102580998283585No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8810.11704032776605751No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8710.11571183369379805No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT8700.11557898428657212No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCGCTGGGAGCAG8670.11518043606489428No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTCCCA8640.11478188784321644No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8300.11026500799753432No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAG8280.10999930918308243No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCT8180.10867081511082297No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTC8160.10840511629637108No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8110.10774086926024136No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC8100.10760801985301542No Hit
GTGTATTACTGTGCGAGATCCCGTTATAGAGATGGTGGAGTGCTTATTATCGGTGATGTTTATGATGTCTGGGGC7930.10534957993017435No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7880.10468533289404462No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC7860.10441963407959273No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7700.10229404356397762No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7690.10216119415675166No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7540.1001684530483625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)