FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697491
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTACTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT66220.39010516108774657No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60430.3559959964441638No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55520.32707095354261084No Hit
GACTTACACTGGTACCAGCAGAAACCACATCAGTCTCCAAAGCTCCTCAT48890.2880133090543632No Hit
GTACTACTCTGATGACAGTAATAGGTTGCAGCATCTTCAGCTTCCAGATTATTGATGGTGAGGGTGAAATCTGTC47900.28218117209457955No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG44460.2619159689211902No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42830.25231356160356666No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35720.21042821434693912No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35170.20718813825817045No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33930.19988323943985567No Hit
CTATTACTGTCATCAGAGTAGTACTTTACCATTCACTTTCGGCCCTGGGA33230.19575950623596824No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA31350.18468433705981357No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31320.1845076056367898No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31280.18427196373942484No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31270.18421305326508358No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30720.18097297717631491No Hit
GTAAAGTACTACTCTGATGACAGTAATAGGTTGCAGCATCTTCAGCTTCCAGATTATTGATGGTGAGGGTGAAAT30490.17961803626646622No Hit
CAGTAATAGGTTGCAGCATCTTCAGCTTCCAGATTATTGATGGTGAGGGT30320.17861655820266498No Hit
TCTCAGGGGTCCCCTCGAGGTTCAATGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAATCTGGAAG30280.1783809163053No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30170.17773290108754627No Hit
GTACTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGA29860.17590667638296756No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29430.17337352598629388No Hit
GATTTCACCCTCACCATCAATAATCTGGAAGCTGAAGATGCTGCAACCTATTACTGTCATCAGAGTAGTACTTTA28900.17025127084620773No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28070.1653617014758841No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26960.15882263882400555No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26240.15458108467143566No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC26150.15405089040236442No Hit
ACCTATTACTGTCATCAGAGTAGTACTTTACCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAA25970.15299050186422194No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25670.1512231876339845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25520.15033953051886578No Hit
TCCCAGCCCTTCTCAGGGGTCCCCTCGAGGTTCAATGGCAGTGGATCTGG24430.1439182888156697No Hit
GTGTAAGTCACTACCAATGGTCTGACTGGCCCGGCAGGTGATGGTGACTT24370.14356482596962222No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCC23740.1398534660861236No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG23660.1393821822913936No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22930.13508171766448246No Hit
CTCCAGGGGTGAGATTGTGCTGACTCAGTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCAC22220.13089907398625383No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22070.1300154168711351No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21770.12824810264089767No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21430.12624514651329521No Hit
GTAATAGGTTGCAGCATCTTCAGCTTCCAGATTATTGATGGTGAGGGTGA21400.12606841509027145No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21180.124772384654764No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20880.12300507042452655No Hit
CCCCTGAGAAGGGCTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGA20830.12271051805282031No Hit
GGATTGGATTGGGTACATCCATCACAATGGGAGCACCAACTACAACCCCTCTTTCAAGAGTCGAGTCACCATAT20660.1217090399890191No Hit
CTCCAAAGCTCCTCATCAAGTATGCTTCCCAGCCCTTCTCAGGGGTCCCC20330.1197649943357579No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20200.11899915816932166No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20140.11864569532327417No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19940.1174674858364492No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19790.11658382872133048No Hit
GACTTACAGCAGCTCGTCAGGAAAGACCTTTGACTACTGGGGCCAGGGAA19610.115523440183188No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT18810.11081060223588815No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18790.11069278128720565No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT18550.10927892990301569No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18240.107452705198437No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT17880.10533192812215206No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17860.10521410717346956No Hit
GTTCAATGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAATC17650.10397698721230333No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG17530.10327006152020836No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17520.1032111510458671No Hit
CCATTGGTAGTGACTTACACTGGTACCAGCAGAAACCACATCAGTCTCCA17450.10279877772547837No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17420.10262204630245463No Hit
GACCATTGGTAGTGACTTACACTGGTACCAGCAGAAACCACATCAGTCTCCAAAGCTCCTCATCAAGTATGCTTC17400.10250422535377213No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17360.10226858345640713No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG17340.10215076250772463No Hit
GCTGTAAGTCCTCGCACAATAATAAACGGCCGTGTCTGCGGCGGTCACAG17240.10156165776431215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)